Phosphate → Acridine Orange Ethidium Bromide

HRID 2264122

反応式

HRID 2264122 の反応式
[CH3:1][N:2]([C:4]1[CH:5]=[CH:6][C:7]2[CH:8]=[C:9]3[C:14](=[N:15][C:16]=2[CH:17]=1)[CH:13]=[C:12]([N:18]([CH3:20])[CH3:19])[CH:11]=[CH:10]3)[CH3:3].[CH3:21][CH2:22][N+:23]1[C:36]([C:37]2[CH:38]=[CH:39][CH:40]=[CH:41][CH:42]=2)=[C:35]2[C:30]([CH:31]=[CH:32][C:33]([NH2:43])=[CH:34]2)=[C:29]2[C:24]=1[CH:25]=[C:26]([NH2:44])[CH:27]=[CH:28]2.[Br-:45].P([O-])([O-])([O-])=O>>[CH3:3][N:2]([C:4]1[CH:5]=[CH:6][C:7]2[CH:8]=[C:9]3[C:14](=[N:15][C:16]=2[CH:17]=1)[CH:13]=[C:12]([N:18]([CH3:20])[CH3:19])[CH:11]=[CH:10]3)[CH3:1].[CH3:21][CH2:22][N+:23]1[C:36]([C:37]2[CH:42]=[CH:41][CH:40]=[CH:39][CH:38]=2)=[C:35]2[C:30]([CH:31]=[CH:32][C:33]([NH2:43])=[CH:34]2)=[C:29]2[C:24]=1[CH:25]=[C:26]([NH2:44])[CH:27]=[CH:28]2.[Br-:45]

参加物

4

反応物 3

HCID 1061PhosphateHCID 14710Ethidium Bromide2 回HCID 62344Acridine Orange Base2 回

生成物 1

HCID 86616552Acridine Orange Ethidium Bromide

実験手順

Apoptosis was determined morphologically after staining with acridine orange and ethidium bromide and counting viable cells by fluorescence microscopy. Cell density was adjusted to 1×106 cells/ml in buffer. Acridine orange and ethidium bromide (1:1) (v/v) mixed in PBS (phosphate buffer solution) to a final concentration of 100 μg/ml. 2 μl dye was mixed with 25 μl of cell suspension and cells were immediately checked.

後処理

  1. addition2 μl dye was mixed with 25 μl of cell suspension and cells

出典と根拠

ORDレコード
ord-0d3710390add475597040889875ff99e
出典データセット
uspto-grants-2010_12
特許番号
US07855073B2

関連ノート

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