Tris(hydroxymethyl)aminomethane → Retinol Palmitate

HRID 230979

反応式

HRID 230979 の反応式
[CH3:1][C:2]1[CH2:7][CH2:6][CH2:5][C:4]([CH3:9])([CH3:8])[C:3]=1[CH:10]=[CH:11][C:12]([CH3:23])=[CH:13][CH:14]=[CH:15][C:16]([CH3:22])=[CH:17][CH2:18][O:19][CH:20]=[O:21].C[C:25]1[CH2:30][CH2:29][CH2:28][C:27](C)([CH3:31])[C:26]=1/[CH:33]=[CH:34]/[C:35](/C)=[CH:36]/[CH:37]=[CH:38]/[C:39](/C)=[CH:40]/CO.CC1CCCC(C)(C)C=1/C=C/C(/C)=C/C=C\C(\C)=C\CO.C(O)C(N)(CO)CO.SC[C@H]([C@@H](CS)O)O.CCCCCCCCCCCCCCCC(OC[C@@H](OC(CCCCCCCCCCCCCCC)=O)COP(OCC[N+](C)(C)C)([O-])=O)=O>>[CH3:40][CH2:39][CH2:38][CH2:37][CH2:36][CH2:35][CH2:34][CH2:33][CH2:26][CH2:25][CH2:30][CH2:29][CH2:28][CH2:27][CH2:31][C:20]([O:19][CH2:18]/[CH:17]=[C:16](/[CH:15]=[CH:14]/[CH:13]=[C:12](/[CH:11]=[CH:10]/[C:3]1[C:4]([CH3:8])([CH3:9])[CH2:5][CH2:6][CH2:7][C:2]=1[CH3:1])\[CH3:23])\[CH3:22])=[O:21]

参加物

7

反応物 6

HCID 6503Tris(hydroxymethyl)aminomethaneHCID 445354Retinol2 回HCID 446094D-1,4-DithiothreitolHCID 452110Colfosceril PalmitateHCID 528038211-cis-Retinol2 回HCID 25058155[3,7-Dimethyl-9-(2,6,6-trimethylcyclohexen-1-yl)nona-2,4,6,8-tetraenyl] formate

生成物 1

HCID 5280531Retinol Palmitate

実験手順

Isomeric preference of the mRPE65/tLRAT mediated esterification of retinols: The effect of mRPE65 on the processing of 11-cis-and all-trans-retinols was determined by monitoring the formation of tLRAT catalyzed retinyl esters from added all-trans-retinol [11,12-3H2], 11-cis-retinol [15-3H] and mRPE65. The reaction mixture (volume 0.1 mL) contains 100 mM Tris (pH 8.4), 5 μM of tLRAT 0.3% CHAPS, 1 mM dithiothreitol and 0.2 μm of all-trans-retinol [11,12-3H2] or 11-cis-retinol [15-3H] and mRPE65 (0.02 μM) or 200 μM/0.4% DPPC/BSA was incubated for 10 min at room temperature. After 10 min the reaction was quenched with 500 μL methanol, 100 μL of water and 500 μL of hexane. The amount of retinyl palmitate formed, as determined by normal phase-HPLC, was used as a measure of activity. Each experiment was done in triplicate, and the data points used are an average of these three points. The standard error was presented as error bars.

後処理

  1. customAfter 10 min the reaction was quenched with 500 μL methanol, 100 μL of water and 500 μL of hexane

出典と根拠

ORDレコード
ord-857f034ffc68496fba840bb435b2c79a
出典データセット
uspto-grants-2009_07
特許番号
US07566808B2

関連ノート

読み込み中…