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Pyruvate → 9-Azido-9-deoxy-N-acetylneuraminic acid
関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|---|---|---|
| 反応物 | Pyruvate | HCID 107735 | — |
| 反応物 | N-Acetyl-Neuraminic Acid | HCID 439197 | — |
| 反応物 | D-1,4-Dithiothreitol | HCID 446094 | — |
| 反応物 | 6-azido-6-deoxy-N-acetylmannosamine | HCID 11708572 | — |
| 溶媒 | Potassium Phosphate, Tribasic | HCID 62657 | — |
| 生成物 | 9-Azido-9-deoxy-N-acetylneuraminic acid | HCID 15479030 | 84% |
反応手順
In a 10 mL of 0.1M potassium phosphate buffer (pH 7.5) containing 10 mM DTT and 0.5M pyruvate were dissolved 50 mg of 6-azido-6-deoxy-N-acetylmannosamine and 1.5 mg of NeuAc aldolase. The starting material was consumed in 14 hours. The solution was lyophilized and the purification was carried out with Bio Gel P-2 gel filtration (3×90 cm) chromatography with a flow rate of 6 mL/40 minutes, at 4° C. The fractions containing the product were pooled and freezed dry to give Compound 203 in 84 percent yield.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 特許番号 | US05593887 |
| 出典データセット | uspto-grants-1997_01 |
| ORD ID | ord-20091454213a4006a46762a1aa23be0d |
| 出典メモ | uspto-grants-1997_01 |