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HRID233556

反应详情

EQUATION

反应方程式

HRID 233556 的结构方程式

REACTANTS

反应物

4
结构：CC(=O)C(=O)[O-]
HCID107735

Pyruvate

C3H3O3-

结构：CC(=O)N[C@H]1[C@H]([C@H](O)[C@H](O)CO)OC(O)(C(=O)O)C[C@@H]1O
HCID439197

N-Acetyl-Neuraminic Acid

C11H19NO9

结构：O[C@H](CS)[C@H](O)CS
HCID446094

Dithiothreitol

C4H10O2S2

结构：CC(=O)N[C@@H]1C(O)O[C@H](CN=[N+]=[N-])[C@@H](O)[C@@H]1O
HCID11708572

未命名化合物

PRODUCTS

生成物

1
结构：CC(=O)N[C@H]1[C@H]([C@H](O)[C@H](O)CN=[N+]=[N-])OC(O)(C(=O)O)C[C@@H]1O
HCID15479030

未命名化合物

收率 84%

AUXILIARIES

试剂、催化剂与溶剂

1
结构：O=P([O-])([O-])[O-].[K+].[K+].[K+]
溶剂HCID62657

Tripotassium phosphate

K3O4P

PROCEDURE

实验过程

In a 10 mL of 0.1M potassium phosphate buffer (pH 7.5) containing 10 mM DTT and 0.5M pyruvate were dissolved 50 mg of 6-azido-6-deoxy-N-acetylmannosamine and 1.5 mg of NeuAc aldolase. The starting material was consumed in 14 hours. The solution was lyophilized and the purification was carried out with Bio Gel P-2 gel filtration (3×90 cm) chromatography with a flow rate of 6 mL/40 minutes, at 4° C. The fractions containing the product were pooled and freezed dry to give Compound 203 in 84 percent yield.

WORKUP

后处理

  1. customThe starting material was consumed in 14 hours
  2. customthe purification
  3. filtrationwas carried out with Bio Gel P-2 gel filtration (3×90 cm) chromatography with a flow rate of 6 mL/40 minutes, at 4° C
  4. additionThe fractions containing the product
  5. customfreezed dry