[NH2:1][C:2]([NH2:4])=[O:3].[C:5]([OH:8])(=[O:7])[CH3:6].[C:9]([NH2:13])(=[O:12])[CH:10]=[CH2:11].CCCCCCCCCCCCOS([O-])(=O)=O.[Na+].C(NC(=O)C=C)NC(=O)C=C.[CH3:43][N:44]([CH2:46][CH2:47][N:48]([CH3:50])[CH3:49])[CH3:45]>O.NC(N)=O.C(O)(=O)C>[C:9]([NH2:13])(=[O:12])[CH:10]=[CH2:11].[C:5]([OH:8])(=[O:7])[CH3:6].[CH3:43][N:44]([CH2:46][CH2:47][N:48]([CH3:50])[CH3:49])[CH3:45].[NH2:1][C:2]([NH2:4])=[O:3]In some experiments, an urea/acetic acid-PAGE system (Panyim, S., and Chalkley, R. (1969) High resolution acrylamide gel electrophoresis of histories. Arch. Biochem. Biophys. 130, 337-346) was used instead of SDS-PAGE to compare the effects of SDS on the capacity of the mAbs to detect the heparin lyases in Western blots. Stock solutions used in the preparation of the urea/acetic acid-PAGE gels were prepared as follows. A 60% acrylamide solution was prepared by dissolving 60 g acrylamide and 0.4 g N,N′-methylene bisacrylamide in 1 00 mL of distilled water. A 43.2% acetic acid/TEMED stock solution was prepared by mixing 43.2 mL acetic acid, 4.0 mL TEMED and 52.8 mL distilled water. APS/urea was prepared by dissolving 5 mg APS in 25 mL of 1 0M urea.
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