Ethanol → Dopamine

HRID 247013

反応式

HRID 247013 の反応式
O=C(O)CN([CH2:8][CH2:9][OH:10])CCO.N[CH2:13][C:14]([OH:16])=O.[CH3:17][C:18]#[N:19].O.[CH2:21](O)[CH3:22]>[Fe-3](C#N)(C#N)(C#N)(C#N)(C#N)C#N.[K+].[K+].[K+]>[NH2:19][CH2:18][CH2:17][C:21]1[CH:22]=[CH:8][C:9]([OH:10])=[C:14]([OH:16])[CH:13]=1

参加物

7

反応物 5

HCID 702EthanolHCID 750GlycineHCID 962WaterHCID 6342AcetonitrileHCID 8761Bicine

生成物 1

HCID 681Dopamine

試薬・触媒・溶媒 1

HCID 178511488HCID 178511488、化合物触媒

実験手順

To the sample solution (1.0 ml) were added 2.0 ml of 0.05M Bicine buffer solution (pH 7.0) containing 0.5M glycine, 0.1 ml of 0.1M DPE solution (ethanol solvent) and 0.1 ml of 0.08% (W/V) potassium ferricyanide solution in sequence. After allowing to stand for more than 30 minutes at 37° C., 100 μl of the reaction liquid were injected into HPLC apparatus. Conditions of HPLC were as follows: column; Ultrasphere-ODS (4.6φ×150 mm, Beckman), eluate; CH3CN--H2O (47.5:52.5 W/V), flow rate 1 ml/min. For the fluorescence detection, Shimazu FLD-I (excitation 300-400 nm, fluorescence 450-800 nm) was employed. Under these conditions, reversed-phase partition type column as mentioned above, norepinephrine, epinephrine and isoproterenol gave a single peak respectively. Although dopamine gave two peaks of an intensity ratio of 9:1, the determination was possible with either of them. The elution was completed within 12 minutes, and the lower limit of detection was 10 pg at S/N ratio of 2.

後処理

  1. waitto stand for more than 30 minutes at 37° C.
  2. custom100 μl of the reaction liquid
  3. customUnder these conditions, reversed-phase partition type column
  4. customgave a single peak respectively

出典と根拠

ORDレコード
ord-3a7df77a6d0442cda7f637b74fb6ea55
出典データセット
uspto-grants-1987_11
特許番号
US04705757

関連ノート

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