化工社化工社 化学情報印刷レポート · 研究参考用
Nadh → Cotinine
関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|---|---|---|
| 反応物 | Nadh | HCID 439153 | — |
| 反応物 | Beta-D-Glucose 6-phosphate | HCID 439427 | — |
| 反応物 | Cotinine | HCID 854019 | — |
| 生成物 | Cotinine | HCID 854019 | — |
| 生成物 | glucose-6-phosphate NAD | HCID 131719436 | — |
反応手順
Cotinine-enzyme conjugate was prepared at 1 mg/ml of protein and diluted 500-fold to a final concentration of 2.0 μg/ml. The anti-cotinine antibody was present in stock solution at an approximate concentration of 15 mg/ml. The glucose-6-phosphate-NAD solution was prepared by mixing 3 volumes of 0.11M glucose-6-phosphate with 2 volumes of 0.1M NAD.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 特許番号 | US05527686 |
| 出典データセット | uspto-grants-1996_06 |
| ORD ID | ord-81ea2360901b4ab6ad533dc50ccdf0ca |
| 出典メモ | uspto-grants-1996_06 |