huagongshe.com
HRID260252

反应详情

EQUATION

反应方程式

HRID 260252 的结构方程式

REACTANTS

反应物

3
结构：NC(=O)C1=CN([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](O)[C@@H]3O)[C@@H](O)[C@H]2O)C=CC1
HCID439153

Adenosine 5'-(trihydrogen diphosphate), P'→5'-ester with 1,4-dihydro-1-.beta.-D-ribofuranosyl-3-pyridinecarboxamide

C21H29N7O14P2

结构：O=P(O)(O)OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O
HCID439427

beta-D-Glucopyranose 6-phosphate

C6H13O9P

结构：CN1C(=O)CC[C@H]1c1cccnc1
HCID854019

Cotinine

C10H12N2O

PRODUCTS

生成物

2
结构：CN1C(=O)CC[C@H]1c1cccnc1
HCID854019

Cotinine

C10H12N2O

结构：NC(=O)C1=CN([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](O)[C@@H]3O)[C@@H](O)[C@H]2O)C=CC1.O=P(O)(O)OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O
HCID131719436

未命名化合物

PROCEDURE

实验过程

Cotinine-enzyme conjugate was prepared at 1 mg/ml of protein and diluted 500-fold to a final concentration of 2.0 μg/ml. The anti-cotinine antibody was present in stock solution at an approximate concentration of 15 mg/ml. The glucose-6-phosphate-NAD solution was prepared by mixing 3 volumes of 0.11M glucose-6-phosphate with 2 volumes of 0.1M NAD.

WORKUP

后处理

  1. additiondiluted 500-fold to a final concentration of 2.0 μg/ml