Fluorescein 5-isothiocyanate → 2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid
反応式
C[C@@H](NC)[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O[C@H]3OC[C@@](O)(C)[C@H](NC)[C@H]3O)[C@H](N)C[C@@H]2N)[C@H](N)CC1.[CH:34]1[C:39](N=C=S)=[CH:38][C:37]2[C:43]([O:45][C:46]3([C:56]4[CH:57]=[CH:58][C:59]([OH:61])=[CH:60][C:55]=4[O:54][C:48]4[CH:49]=[C:50]([OH:53])[CH:51]=[CH:52][C:47]3=4)[C:36]=2[CH:35]=1)=[O:44]>C(=O)([O-])[O-].[Na+].[Na+].C(=O)(O)[O-]>[CH:34]1[CH:39]=[CH:38][C:37]([C:43]([OH:45])=[O:44])=[C:36]([C:46]2[C:47]3[CH:52]=[CH:51][C:50]([OH:53])=[CH:49][C:48]=3[O:54][C:55]3[C:56]=2[CH:57]=[CH:58][C:59]([CH:60]=3)=[O:61])[CH:35]=1参加物
4
実験手順
Gentamicin (1mM) and fluorescein isothiocyanate (FITC) (1.25mM) were allowed to react in 0.05M sodium carbonate/bicarbonate buffer, pH 9.0, for 2 hours at room temperature. 2ml of the reaction mixture was applied to a column (1 × 97cm) of G-15 grade Sephadex and eluted with carbonate/bicarbonate buffer as above, with a flow rate of 1.8ml/h. Column fractions (1.8ml) were assayed for gentamicin content by radio-immunoassay and for fluorescein content by fluorimetry. FIG. 1 of the accompanying drawings shows the result. Unreacted gentamicin left the column first. The FTC-G product exited next, well separated from the gentamicin peak. Unreacted FITC was very strongly held on the G-15 column and was not eluted.