反応式
C1N=C(N)C2N=CN([C@@H]3O[C@H](COP([O:20][P:21]([O:24][CH2:25][C@H:26]4[O:30][C@@H:29](N5C=C(C(N)=O)CC=C5)[C@H:28]([OH:40])[C@@H:27]4[OH:41])([OH:23])=[O:22])(O)=O)[C@@H](O)[C@H]3O)C=2N=1.[OH-:45].[Na+].C[CH2:48][O:49]CCOCCO>>[CH2:25]([O:24][P:21]([OH:23])([OH:20])=[O:22])[C@H:26]1[O:30][C@@H:29]([OH:45])[C@H:28]([OH:40])[C@@H:27]([OH:41])[C@@H:48]1[OH:49]参加物
実験手順
To 0.5 ml G-6-PDH (2.5 mg/ml, 777 lV/mg) was added 20 mg G-6-PNa2, 20 mg NADH, 150 μl of carbitol and the NHS ester prepared above in 3 1 μl aliquots followed by a 0.05 μl aliquot over a period of about 3 hrs while maintaining the pH with 0.1 N NaOH between 8.55-8.77, to provide an amitriptyline-enzyme mole ratio of about 58.3. The reaction mixture was then chromatographed over Sephadex G-50 M (80 ml column) employing 0.05 M carbonate buffer (pH9.6) as eluent, collecting 20 drop fractions with fractions 16-21 pooled to provide a total volume of 8 ml. The max rate as determined by a G-6-PDH assay to be described was ΔOD/min 653.3 (% deactivation 82.8) and % inhibitability using saturating amounts of anti(amitriptyline) varied from 85.3 to 90.8 depending on the source of antibody.
出典と根拠
- ORDレコード
- ord-18ab8da181ed4962874893c1271ae8a4
- 出典データセット
- uspto-grants-1980_09
- 特許番号
- US04223013
関連ノート
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