Ninhydrin → 6-Carboxyfluorescein succinimidyl ester
反応式
[CH:1]1[C:6]([C:7]([OH:9])=[O:8])=[CH:5][C:4]2[C:10]3([O:26][C:27](=[O:28])[C:3]=2[CH:2]=1)[C:20]1[CH:21]=[CH:22][C:23]([OH:25])=[CH:24][C:19]=1[O:18][C:12]1[CH:13]=[C:14]([OH:17])[CH:15]=[CH:16][C:11]3=1.C1C=[C:33]2[C:35](C(O)(O)[C:38](=[O:39])[C:32]2=CC=1)=[O:36].CC[N:44](C(C)C)C(C)C.CN(C=O)C>>[CH2:32]1[C:38](=[O:39])[N:44]([O:8][C:7]([C:6]2[CH:1]=[CH:2][C:3]3[C:27]([O:26][C:10]4([C:20]5[CH:21]=[CH:22][C:23]([OH:25])=[CH:24][C:19]=5[O:18][C:12]5[CH:13]=[C:14]([OH:17])[CH:15]=[CH:16][C:11]4=5)[C:4]=3[CH:5]=2)=[O:28])=[O:9])[C:35](=[O:36])[CH2:33]1参加物
4
実験手順
The resin was treated with 2 equivalents 6-FAM-NHS in 1% DIEA/DMF and stirred or shaken at ambient temperature overnight. When complete, the resin was drained, washed thrice with DMF, thrice with (1× dichloromethane and 1× methanol) and dried to provide an orange resin that was negative by ninhydrin test.
後処理
- stirringshaken at ambient temperature overnight
- washwashed thrice with DMF, thrice with (1× dichloromethane and 1× methanol)