反応式
CCC[CH2:4][CH2:5][CH2:6][CH2:7][CH2:8][CH2:9][CH2:10][CH2:11][CH2:12][O:13]S([O-])(=O)=O.[Na+].[SiH4]>>[CH:12](=[O:13])[CH2:11][CH2:10][CH2:9][CH2:8][CH2:7][CH2:6][CH2:5][CH3:4]参加物
3
実験手順
Targets are complementary oligonulceotides labeled with fluorescent dye Cy3. Slides were hybridized with targets in hybridization buffer (2×SSC, 0.1% SDS and complementary target at 100 nM) at room temperature for 1 hr and washed in 2×SSC, 0.1% SDS for 5 min., 0.1×SSC, 0.1% SDS for 10 min., and finally in 0.1×SSC for 1 min. All washings were carried out at room temperature. Results show that the aldehyde slides prepared from photoactivatable silane gave much higher hybridization signal than non-aldehyde slides.
後処理
- washwashed in 2×SSC, 0.1% SDS for 5 min.
- waitfinally in 0.1×SSC for 1 min
- customwere carried out at room temperature
- customResults
出典と根拠
- ORDレコード
- ord-89c4572ca97d435ab49ba8ac02e6b6f6
- 出典データセット
- uspto-grants-2007_02
- 特許番号
- US07176297B2
関連ノート
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