Nitrous Acid → 3-Methyl-2-benzothiazolone hydrazone
反応式
N(O)=O.Cl.[CH3:5][N:6]1[C:10]2[CH:11]=[CH:12][CH:13]=[CH:14][C:9]=2[S:8][C:7]1=[N:15][NH2:16].OC1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1N>>[CH3:5][N:6]1[C:10]2[C:9]([S:8]/[C:7]/1=[N:15]\[NH2:16])=[CH:14][CH:13]=[CH:12][CH:11]=2参加物
4
実験手順
Nitrous acid oxidation followed by reaction with 3-methyl-2-benzothiazolone hydrazone hydrochloride (MBTH) and then FeCl3 was used to measure the number of free amino groups (equivalent to the number of glucosamines) in the glycolchitosan and derived products (Davies et al., Meth. Enzymol. 161:442-446 (1988)). The assay was standardized with glucosamine on a weight basis. Also the MBTH/Fe3+ stage of this procedure was used to measure the number of aldehyde groups, similarly relying on standardization with glucosamine treated with nitrous acid.
出典と根拠
- ORDレコード
- ord-4f9855f6bfe048f0b7fd8b36d40e0492
- 出典データセット
- uspto-grants-1995_05
- 特許番号
- US05412083
反応ノート
読み込み中…