Methane → Trehalose

HRID 375061

反応式

HRID 375061 の反応式
[CH2:1]([OH:23])[C@H:2]1[O:7][C@H:6]([O:8][C@:9]2([CH2:18][OH:19])[O:13][C@H:12]([CH2:14][OH:15])[C@@H:11]([OH:16])[C@@H:10]2[OH:17])[C@H:5]([OH:20])[C@@H:4]([OH:21])[C@@H:3]1[OH:22].C>>[CH2:1]([OH:23])[C@H:2]1[O:7][C@H:6]([O:8][C@H:9]2[O:13][C@H:12]([CH2:14][OH:15])[C@@H:11]([OH:16])[C@H:10]([OH:17])[C@H:18]2[OH:19])[C@H:5]([OH:20])[C@@H:4]([OH:21])[C@@H:3]1[OH:22]

参加物

3

反応物 2

HCID 297MethaneHCID 5988Sucrose

生成物 1

HCID 7427Trehalose

反応条件

温度
5 °C

実験手順

Incubation of Brevibacterium lactofermentum ATCC 13869 was effected in the same manner as in Example 1, except that sucrose was used as the carbon source. One liter of the culture obtained was sterilized, then treated with an anion exchange resin AMBERLITE IR-4B and a cation exchange resin AMBERLITE IR-120 and decolored with active charcoal. Next, the decolored liquid was concentrated to about 25 ml, and 75 ml of ethanol was added thereto and left to cool to 5° C. to obtain 8.5 g of trehalose crystals having a purity of 99%.

後処理

  1. customOne liter of the culture obtained
  2. additiontreated with an anion exchange resin AMBERLITE IR-4B
  3. concentrationNext, the decolored liquid was concentrated to about 25 ml, and 75 ml of ethanol
  4. additionwas added
  5. waitleft

出典と根拠

ORDレコード
ord-b2918bc31bf04b3691d63fbb0871c0b9
出典データセット
uspto-grants-1996_01
特許番号
US05484714

関連ノート

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