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Bicarbonate Ion → ((2R,3S,4R,5R)-5-(6-Amino-8-((2-aminoethyl)amino)-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl dihydrogen phosphate

HRID 414163
ノート
印刷プレビュー

反応式

HRID 414163 の反応式
[P:1]([O:5][CH2:6][C@H:7]1[O:11][C@@H:10]([N:12]2[C:21]3[N:20]=[CH:19][N:18]=[C:16]([NH2:17])[C:15]=3[N:14]=[C:13]2Br)[C@H:9]([OH:23])[C@@H:8]1[OH:24])([OH:4])([OH:3])=[O:2].[CH2:25]([NH2:28])[CH2:26][NH2:27].[OH-].[Na+].C(=O)(O)[O-]>O>[P:1]([O:5][CH2:6][C@H:7]1[O:11][C@@H:10]([N:12]2[C:21]3[N:20]=[CH:19][N:18]=[C:16]([NH2:17])[C:15]=3[N:14]=[C:13]2[NH:27][CH2:26][CH2:25][NH2:28])[C@H:9]([OH:23])[C@@H:8]1[OH:24])([OH:4])([OH:3])=[O:2]
参加物実験手順後処理出典と根拠反応ノート

参加物

6

反応物 4

HCID 769Bicarbonate IonHCID 3301EthylenediamineHCID 14798Sodium HydroxideHCID 1681208-Bromo-9-(5-O-phosphonopentofuranosyl)-9H-purin-6-amine

生成物 1

HCID 22823186((2R,3S,4R,5R)-5-(6-Amino-8-((2-aminoethyl)amino)-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl dihydrogen phosphate

試薬・触媒・溶媒 1

HCID 962
Water
溶媒

実験手順

A reaction mixture consisting of 2.2 mmol 8-bromoadenosine-5'-monophosphate, prepared according to the method described in Arch. Biochem. Biophys. 163: 561-9(1974), 66 mmol ethylenediamine and 25 ml water was heated in an oil bath at 140° for 2 hr. The cooled mixture was adjusted to pH 11.5 with sodium hydroxide and passed into a 2.5×55 cm column of Dowex 1×8 (200-400 mesh, bicarbonate form). The column was washed with 300 ml water and then with a linear gradient generated with 3 liters water and 3 liters 0.5M ammonium bicarbonate. The absorbance of the effluent at 254 nm was monitored and a major peak of absorbing material eluted between 4.6 and 5.8 liters of the gradient.

後処理

  1. customprepared
  2. washThe column was washed with 300 ml water
  3. washa major peak of absorbing material eluted between 4.6 and 5.8 liters of the gradient

出典と根拠

ORDレコード
ord-abfc018b68cc4b24ab2fa6fd05fcb8c1
出典データセット
uspto-grants-1988_12
DOI
10.6084/m9.figshare.5104873.v1
特許番号
US04791055

反応ノート

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