Staurosporine → Biphenyl

反応式

CN[C@@H]1C[C@H]2O[C@@](C)([C@@H]1OC)n1c3ccccc3c3c4c(c5c6ccccc6n2c5c31)C(=O)NC4.N#Cc1ccc(C=O)cc1Br>CC(=O)N(C)C.CC(=O)O.CC(=O)O[BH-](OC(C)=O)OC(C)=O.CC(C)[O-].CC(C)[O-].CC(C)[O-].CC(C)[O-].[Na+].[Ti+4]>CO[C@@H]1[C@H](N(C)Cc2ccc(C#N)c(Br)c2)C[C@H]2O[C@]1(C)n1c3ccccc3c3c4c(c5c6ccccc6n2c5c31)C(=O)NC4

参加物

反応条件

温度: 22 °C

後処理

additionThe next morning, the Mosquito(R) was used to dilute the 100 mM reactions to 50 mM via addition of 1 uL DMSO to each reaction well, followed by dispense mixing.
additionThe reactions were diluted with 3x1 uL DMSO (containing PhPh as an internal standard) using the Mosquito(R). The reactions were mixed after each dispense operation.
aliquotMosquito(R) was used to transfer 1 uL of each quenched reaction from the 1,536-well reaction plate to 384-well analytical plates containing 60 uL DMSO for analysis by UPLC.
additionMosquito(R) was used to transfer 1 uL of each quenched reaction from the 1,536-well reaction plate to 384-well analytical plates containing 60 uL DMSO for analysis by UPLC.

出典と根拠

ORDレコード: ord-6159d1a90187472bb0285fa0b9da0e71
出典データセット: Cernak miniaturized reductive amination
DOI: 10.1038/s44160-023-00351-1
元リンク: 出典を表示

反応ノート

読み込み中…

参加物

反応物 2

HCID 44259Staurosporine

HCID 534255192-Bromo-4-formylbenzonitrile

生成物 4

HCID 7095Biphenyl

HCID 44259Staurosporine

HCID 2884342-Bromo-4-(hydroxymethyl)benzonitrile

HCID 178496679HCID 178496679、化合物

試薬・触媒・溶媒 4

HCID 176Acetic Acid試薬

HCID 11026Titanium isopropoxide試薬

HCID 5049666Sodium triacetoxyborohydride試薬

HCID 31374Dimethylacetamide溶媒7 回

反応条件

温度: 22 °C

後処理

additionThe next morning, the Mosquito(R) was used to dilute the 100 mM reactions to 50 mM via addition of 1 uL DMSO to each reaction well, followed by dispense mixing.
additionThe reactions were diluted with 3x1 uL DMSO (containing PhPh as an internal standard) using the Mosquito(R). The reactions were mixed after each dispense operation.
aliquotMosquito(R) was used to transfer 1 uL of each quenched reaction from the 1,536-well reaction plate to 384-well analytical plates containing 60 uL DMSO for analysis by UPLC.
additionMosquito(R) was used to transfer 1 uL of each quenched reaction from the 1,536-well reaction plate to 384-well analytical plates containing 60 uL DMSO for analysis by UPLC.

出典と根拠

ORDレコード: ord-6159d1a90187472bb0285fa0b9da0e71
出典データセット: Cernak miniaturized reductive amination
DOI: 10.1038/s44160-023-00351-1
元リンク: 出典を表示

反応ノート

読み込み中…