Staurosporine → Staurosporine

HRID 438805

反応式

HRID 438805 の反応式
CN[C@@H]1C[C@H]2O[C@@](C)([C@@H]1OC)n1c3ccccc3c3c4c(c5c6ccccc6n2c5c31)C(=O)NC4.Cc1noc(C)c1C=O>CC(=O)O.CC(=O)O[BH-](OC(C)=O)OC(C)=O.CC(C)[O-].CC(C)[O-].CC(C)[O-].CC(C)[O-].CN1CCCC1=O.[Na+].[Ti+4]>CO[C@@H]1[C@H](N(C)Cc2c(C)noc2C)C[C@H]2O[C@]1(C)n1c3ccccc3c3c4c(c5c6ccccc6n2c5c31)C(=O)NC4

参加物

9

反応物 2

HCID 44259StaurosporineHCID 2895763,5-Dimethyl-4-Isoxazolecarbaldehyde

生成物 3

HCID 44259StaurosporineHCID 2895763,5-Dimethyl-4-IsoxazolecarbaldehydeHCID 178496690HCID 178496690、化合物

試薬・触媒・溶媒 4

HCID 176Acetic Acid試薬HCID 11026Titanium isopropoxide試薬HCID 5049666Sodium triacetoxyborohydride試薬HCID 13387N-methyl-2-pyrrolidone溶媒7 回

反応条件

温度
22 °C

後処理

  1. additionThe next morning, the Mosquito(R) was used to dilute the 100 mM reactions to 50 mM via addition of 1 uL DMSO to each reaction well, followed by dispense mixing.
  2. additionThe reactions were quenched with 1 uL saturated aqueous NaHCO3.
  3. additionThe reactions were quenched with 1 uL water.
  4. additionThe reactions were diluted to approximately 10 mM.
  5. aliquotMosquito(R) was used to transfer 1 uL of each quenched reaction from the 1,536-well reaction plate to 384-well analytical plates containing 60 uL DMSO for analysis by UPLC.
  6. additionMosquito(R) was used to transfer 1 uL of each quenched reaction from the 1,536-well reaction plate to 384-well analytical plates containing 60 uL DMSO for analysis by UPLC.

出典と根拠

ORDレコード
ord-6b7852ceb09b441698d9b5456995cd1c
出典データセット
Cernak miniaturized reductive amination
元リンク
出典を表示

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