Phosphate → Nadh

HRID 459735

反応式

HRID 459735 の反応式
[CH:1]1[CH:6]=[N+:5]([C@@H:7]2[O:11][C@H:10]([CH2:12][O:13][P:14]([O:17][P:18]([O:21][CH2:22][C@H:23]3[O:27][C@@H:26]([N:28]4[C:32]5[N:33]=[CH:34][N:35]=[C:36]([NH2:37])[C:31]=5[N:30]=[CH:29]4)[C@H:25]([OH:38])[C@@H:24]3[OH:39])([OH:20])=[O:19])([OH:16])=[O:15])[C@@H:9]([OH:40])[C@H:8]2[OH:41])[CH:4]=[C:3]([C:42]([NH2:44])=[O:43])[CH:2]=1.P([O-])([O-])([O-])=O.[OH-].[Na+]>>[CH:34]1[N:35]=[C:36]([NH2:37])[C:31]2[N:30]=[CH:29][N:28]([C@@H:26]3[O:27][C@H:23]([CH2:22][O:21][P:18]([O:17][P:14]([O:13][CH2:12][C@H:10]4[O:11][C@@H:7]([N:5]5[CH:4]=[C:3]([C:42]([NH2:44])=[O:43])[CH2:2][CH:1]=[CH:6]5)[C@H:8]([OH:41])[C@@H:9]4[OH:40])([OH:16])=[O:15])([OH:20])=[O:19])[C@@H:24]([OH:39])[C@H:25]3[OH:38])[C:32]=2[N:33]=1

参加物

4

反応物 3

HCID 1061PhosphateHCID 5893Nadide (JAN/USAN/INN)HCID 14798Sodium Hydroxide

生成物 1

HCID 439153Nadh収率 24.2%

反応条件

温度
23 °C

実験手順

NAD+ (34.0 mg, 38.0 μmol) was placed in 2 mL of a 0.1 M phosphate buffer. The pH of this solution was adjusted to 8.1 with NaOH and then placed in an 80 mL Fisher-Porter bottle. The Fisher-Porter bottle was evacuated and refilled with argon three times then [Cl2Ru(TPPTS)2]2 (6.9 mg, 2.6 μmol) was added under a flow of argon. The Fisher-Porter bottle was evacuated and filled with 70 psi of H2 and stirred to 23° C. for 17.0 hr. The volatiles were removed under reduced pressure and a sample dissolved in D2O To this was added 20 μl of trimethylsilylpropionate standard solution. 1H NMR integration shows that 9.2 μmole of NADH have been produced under these conditions.

後処理

  1. customThe Fisher-Porter bottle was evacuated
  2. additionrefilled with argon three times
  3. customThe Fisher-Porter bottle was evacuated
  4. additionfilled with 70 psi of H2
  5. customThe volatiles were removed under reduced pressure
  6. dissolutiona sample dissolved in D2O To
  7. additionthis was added 20 μl of trimethylsilylpropionate standard solution

出典と根拠

ORDレコード
ord-6f3cfee9981d4a8d933484091f6148f7
出典データセット
uspto-grants-2007_03
特許番号
US07195889B2

関連ノート

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