Nadide (JAN/USAN/INN) → NADPH
反応式
[CH:1]1[N:2]=[C:3]([NH2:44])[C:4]2[N:9]=[CH:8][N:7]([C@@H:10]3[O:14][C@H:13]([CH2:15][O:16][P:17]([O:20][P:21]([O:24][CH2:25][C@H:26]4[O:30][C@@H:29]([N:31]5[CH:36]=[C:35]([C:37]([NH2:39])=[O:38])[CH2:34][CH:33]=[CH:32]5)[C@H:28]([OH:40])[C@@H:27]4[OH:41])([OH:23])=[O:22])([OH:19])=[O:18])[C@@H:12]([OH:42])[C@H:11]3[OH:43])[C:5]=2[N:6]=1.[CH:45]1[CH:50]=[N+:49]([C@@H:51]2[O:55][C@H:54]([CH2:56][O:57][P:58]([O:61][P:62]([O:65][CH2:66][C@H:67]3[O:71][C@@H:70]([N:72]4[C:76]5[N:77]=[CH:78][N:79]=[C:80]([NH2:81])[C:75]=5[N:74]=[CH:73]4)[C@H:69]([OH:82])[C@@H:68]3[OH:83])([OH:64])=[O:63])([OH:60])=[O:59])[C@@H:53]([OH:84])[C@H:52]2[OH:85])[CH:48]=[C:47]([C:86]([NH2:88])=[O:87])[CH:46]=1>>[CH:1]1[N:2]=[C:3]([NH2:44])[C:4]2[N:9]=[CH:8][N:7]([C@@H:10]3[O:14][C@H:13]([CH2:15][O:16][P:17]([O:20][P:21]([O:24][CH2:25][C@H:26]4[O:30][C@@H:29]([N:31]5[CH:36]=[C:35]([C:37]([NH2:39])=[O:38])[CH2:34][CH:33]=[CH:32]5)[C@H:28]([OH:40])[C@@H:27]4[OH:41])([OH:23])=[O:22])([OH:19])=[O:18])[C@@H:12]([OH:42])[C@H:11]3[O:43][P:58]([OH:60])([OH:59])=[O:57])[C:5]=2[N:6]=1.[CH:45]1[CH:50]=[N+:49]([C@@H:51]2[O:55][C@H:54]([CH2:56][O:57][P:58]([O:61][P:62]([O:65][CH2:66][C@H:67]3[O:71][C@@H:70]([N:72]4[C:76]5[N:77]=[CH:78][N:79]=[C:80]([NH2:81])[C:75]=5[N:74]=[CH:73]4)[C@H:69]([O:82][P:17]([OH:19])([OH:18])=[O:16])[C@@H:68]3[OH:83])([OH:64])=[O:63])([OH:60])=[O:59])[C@@H:53]([OH:84])[C@H:52]2[OH:85])[CH:48]=[C:47]([C:86]([NH2:88])=[O:87])[CH:46]=1参加物
4
反応条件
- 温度
- 100 °C
実験手順
To 0.9 ml of each of the above substrate solutions containing NADH or NAD+ was added 0.1 ml of 23 U/ml of the present enzyme, and the reaction was carried out at 30° C. for 20 minutes. Then, the reaction was terminated by heat treatment at 100° C. for 2 minutes, and the denatured protein was removed, after which the amount of NADPH or NADP+ produced by the reaction of the corresponding substrate was measured as follows. As a control solution, there was used a mixture of each substrate solution and the present enzyme in which the reaction had been terminated immediately after mixing them.