Acetic Acid → ethyl 3-(3-((((3aR,4R,6R,6aR)-6-(6-amino-9H-purin-9-yl)-2,2-dimethyltetrahydrofuro[3,4-d][1,3]dioxol-4-yl)methyl)amino)cyclobutyl)propanoate

HRID 511573

反応式

HRID 511573 の反応式
[NH2:1][CH2:2][C@@H:3]1[C@H:7]2[O:8][C:9]([CH3:12])([CH3:11])[O:10][C@H:6]2[C@H:5]([N:13]2[CH:21]=[N:20][C:19]3[C:14]2=[N:15][CH:16]=[N:17][C:18]=3[NH2:22])[O:4]1.O=[C:24]1[CH2:27][CH:26]([CH2:28][CH2:29][C:30]([O:32][CH2:33][CH3:34])=[O:31])[CH2:25]1.C(O)(=O)C.C([BH3-])#N.[Na+]>CO.O>[NH2:22][C:18]1[N:17]=[CH:16][N:15]=[C:14]2[C:19]=1[N:20]=[CH:21][N:13]2[C@H:5]1[C@@H:6]2[O:10][C:9]([CH3:12])([CH3:11])[O:8][C@@H:7]2[C@@H:3]([CH2:2][NH:1][CH:24]2[CH2:25][CH:26]([CH2:28][CH2:29][C:30]([O:32][CH2:33][CH3:34])=[O:31])[CH2:27]2)[O:4]1

参加物

7

反応物 4

HCID 176Acetic AcidHCID 5003444Sodium cyanoborohydrideHCID 113012155'-Amino-5'-deoxy-2',3'-O-(1-methylethylidene)-adenosineHCID 67775333Ethyl 3-(3-oxocyclobutyl)propanoate2 回

生成物 1

HCID 67774002ethyl 3-(3-((((3aR,4R,6R,6aR)-6-(6-amino-9H-purin-9-yl)-2,2-dimethyltetrahydrofuro[3,4-d][1,3]dioxol-4-yl)methyl)amino)cyclobutyl)propanoate

試薬・触媒・溶媒 2

HCID 887Methanol溶媒2 回HCID 962Water溶媒

実験手順

A mixture of 9-((3aR,4R,6R,6aR)-6-(aminomethyl)-2,2-dimethyltetrahydrofuro[3,4-d][1,3]dioxol-4-yl)-9H-purin-6-amine (0.50 g, 1.6 mmol) and ethyl 3-(3-oxocyclobutyl)propanoate (0.27 g, 1.6 mmol) in methanol (10 mL) was treated with acetic acid (0.09 mL, 2 mmol) at rt and the flask was evacuated and flushed with nitrogen (×3). The reaction mixture was treated at rt with sodium cyanoborohydride (0.26 g, 4.1 mmol), which afforded instant gas evolution and a nearly colorless, clear solution in a few minutes. The reaction mixture was stirred for 1 h at rt; HPLC/LC MS indicated a ˜2:1 mixture of product to starting amine. At 1.5 h additional ethyl 3-(3-oxocyclobutyl)propanoate (66 mg, 0.39 mmol) in MeOH (1.0 mL) was added and the reaction mixture was stirred at rt for 30 min; HPLC/LC MS indicated ˜70% conversion and some dialkylation. At 2 h 15 min water (4.0 mL) was added and the mixture was concentrated in vacuo. The residual aqueous layer was diluted with saturated aqueous sodium bicarbonate (10 mL, to pH 9) and extracted with CH2Cl2 (3×15 mL). The combined organics were dried (Na2SO4) and concentrated in vacuo to afford the reductive amination product as a white foam, which was carried on without further purification: MS (ESI+) for C22H32N6O5 m/z 461.1 (M+H)+, 483.1 (M+Na)+.

後処理

  1. customthe flask was evacuated
  2. customflushed with nitrogen (×3)
  3. customafforded instant gas evolution
  4. waita nearly colorless, clear solution in a few minutes
  5. additiona ˜2:1 mixture of product
  6. stirringthe reaction mixture was stirred at rt for 30 min
  7. concentrationthe mixture was concentrated in vacuo
  8. additionThe residual aqueous layer was diluted with saturated aqueous sodium bicarbonate (10 mL, to pH 9)
  9. extractionextracted with CH2Cl2 (3×15 mL)
  10. dry with materialThe combined organics were dried (Na2SO4)
  11. concentrationconcentrated in vacuo

出典と根拠

ORDレコード
ord-d69bc03d0ff54dee9800da2645ab4ef0
出典データセット
uspto-grants-2015_08
特許番号
US09096634B2

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