反応式
ON1C(=O)CCC1=O.[C:9]([OH:40])(=[O:39])[CH2:10][CH2:11][C@H:12]([NH:16][C:17]([C:19]1[CH:38]=[CH:37][C:22]([NH:23][CH2:24][C:25]2[N:36]=[C:35]3[C:28]([N:29]=[C:30]([NH:32][C:33]3=[O:34])[NH2:31])=[N:27][CH:26]=2)=[CH:21][CH:20]=1)=[O:18])[C:13]([OH:15])=[O:14].C1(N=C=NC2CCCCC2)CCCCC1.[NH2:56][CH:57]([NH2:63])[CH2:58][CH2:59][CH2:60][CH2:61][CH3:62]>CS(C)=O>[C:9]([O-:40])(=[O:39])[CH2:10][CH2:11][C@H:12]([NH:16][C:17]([C:19]1[CH:20]=[CH:21][C:22]([NH:23][CH2:24][C:25]2[N:36]=[C:35]3[C:28]([N:29]=[C:30]([NH:32][C:33]3=[O:34])[NH2:31])=[N:27][CH:26]=2)=[CH:37][CH:38]=1)=[O:18])[C:13]([OH:15])=[O:14].[NH2:56][CH:57]([NH2:63])[CH2:58][CH2:59][CH2:60][CH2:61][CH3:62]参加物
実験手順
8 mg NHS (N-Hydroxysuccinimide) was added to 16 mg folic acid in 4 ml DMSO solvent followed by 9 mg of DCC (dicyclohexyl carbodiimide) in 500 μl DMSO. It was then incubated at room temperature for 2 h then added to 4.7 mg Diaminohexane in 500 μl DMSO forming a white-yellow precipitate/suspension. It was left overnight to react to completion forming the folate-diaminohexane linker. 156 μl of this was then added to 1 mg of Sulfo-SBED (2-[(6-(Biotinamido)-2-(p-azidobenzamido)-hexanamido]ethyl-1-3′-dithio-propionate) (Pierce & Warriner product number 33033) in 100 μl DMF and incubated for a further 48 h during which time the solution clarified. This was followed by the addition of 750 μl distilled water to neutralise any tridentate-NHS ester groups which might have remained. After a further 4 h 500 μl of this hydrolysed tridentate-folate complex was added to 1 mg (500 μl) of antibody and incubated for a further 48 h.
後処理
- waitIt was left overnight
- customto react to completion
出典と根拠
- ORDレコード
- ord-e5a01b0619c74b10a603b6eee9a0d010
- 出典データセット
- uspto-grants-2011_11
- 特許番号
- US08062855B2
関連ノート
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