Diethyl Ether → (S)-3-[[(1,1-Dimethylethoxy)carbonyl]amino]-hexanedioic acid 1-methyl 6-(phenylmethyl) ester

温度

0 °C

4-Methylmorpholine (2 ml) was added to a solution of t-butoxycarbonyl-L-glutamic acid 5-benzyl ester (6.75 g,20 mmol) in ethyl acetate (60 ml) at 0° C. i-Butyl chloroformate (2.61 ml,20 mmol) was then added to give a precipitate and the mixture was stirred for 15 min at 0° C. before filtering through celite into a flask at 0° C. The pad was washed with precooled ethyl acetate. The combined filtrate and wash was treated with diazomethane in ether (30 mmol in 100 ml) over 5 min and the reaction left at 0° C. for 3h before warming to room temperature. Excess diazomethane was removed by bubbling nitrogen through the solution which was then concentrated to give a yellow gum. The gum was dissolved in methanol (100 ml), treated with silver benzoate (1 g, 4.4 mmol) and triethylamine (10 ml,70 mmol) then stirred for 2.5h at room temperature. The black solution was concentrated under reduced pressure and the residue taken up in ethyl acetate (100 ml) then washed with saturated aqueous sodium bicarbonate solution (100 ml), water (100 ml), 1M aqueous potassium hydrogen sulphate solution (2×100 ml) and brine (100 ml). The dried (MgSO4) organic phase was evaporated to leave a yellow oil which was purified by column chromatography on flash silica eluting with a gradient of 15-50% ethyl acetate in cyclohexane to give the title compound (1.972 g) as a white solid which crystallised from cyclohexane m.p.60-61° C., [α]D -21° (CHCl3 c=1%).

1. additionwas then added
2. customto give a precipitate
3. filtrationbefore filtering through celite into a flask at 0° C
4. washThe pad was washed with precooled ethyl acetate
5. washThe combined filtrate and wash
6. temperaturebefore warming to room temperature
7. customExcess diazomethane was removed
8. customby bubbling nitrogen through the solution which
9. concentrationwas then concentrated
10. customto give a yellow gum
11. concentrationThe black solution was concentrated under reduced pressure
12. washthen washed with saturated aqueous sodium bicarbonate solution (100 ml), water (100 ml), 1M aqueous potassium hydrogen sulphate solution (2×100 ml) and brine (100 ml)
13. dry with materialThe dried (MgSO4) organic phase
14. customwas evaporated
15. customto leave a yellow oil which
16. customwas purified by column chromatography on flash silica eluting with a gradient of 15-50% ethyl acetate in cyclohexane

ORDレコード

ord-6af57fcec4334f1ab9959ffa05ebb59d

出典データセット

uspto-grants-1999_07

DOI

10.6084/m9.figshare.5104873.v1

特許番号

US05925624