1-Hydroxybenzotriazole → Benzyl (S)-3-(4-methoxyphenyl)-2-((S)-2-(2-morpholinoacetamido)propanamido)propanoate

HRID 58804

反応式

HRID 58804 の反応式
[NH2:1][C@@H:2]([CH3:26])[C:3]([NH:5][C@@H:6]([CH2:17][C:18]1[CH:23]=[CH:22][C:21]([O:24][CH3:25])=[CH:20][CH:19]=1)[C:7]([O:9][CH2:10][C:11]1[CH:16]=[CH:15][CH:14]=[CH:13][CH:12]=1)=[O:8])=[O:4].CN(C(ON1N=NC2C=CC=CC1=2)=[N+](C)C)C.F[P-](F)(F)(F)(F)F.C1C=CC2N(O)N=NC=2C=1.[O:61]1[CH2:66][CH2:65][N:64]([CH2:67][C:68](O)=[O:69])[CH2:63][CH2:62]1.CCN(C(C)C)C(C)C.C([O-])(O)=O.[Na+]>CN(C=O)C>[CH3:25][O:24][C:21]1[CH:20]=[CH:19][C:18]([CH2:17][C@H:6]([NH:5][C:3](=[O:4])[C@@H:2]([NH:1][C:68](=[O:69])[CH2:67][N:64]2[CH2:65][CH2:66][O:61][CH2:62][CH2:63]2)[CH3:26])[C:7]([O:9][CH2:10][C:11]2[CH:16]=[CH:15][CH:14]=[CH:13][CH:12]=2)=[O:8])=[CH:23][CH:22]=1

参加物

8

反応物 6

HCID 757711-HydroxybenzotriazoleHCID 81531DiisopropylethylamineHCID 4389684-Morpholineacetic acidHCID 516892Sodium BicarbonateHCID 2733084Methanaminium, N-((1H-benzotriazol-1-yloxy)(dimethylamino)methylene)-N-methyl-, hexafluorophosphate(1-) (1:1)HCID 130394596(S)-benzyl 2-((S)-2-aminopropanamido)-3-(4-methoxyphenyl)propanoate

生成物 1

HCID 117870627Benzyl (S)-3-(4-methoxyphenyl)-2-((S)-2-(2-morpholinoacetamido)propanamido)propanoate収率 89%

試薬・触媒・溶媒 1

HCID 6228Dimethylformamide溶媒

実験手順

To a solution of (S)-benzyl 2-((S)-2-aminopropanamido)-3-(4-methoxyphenyl)propanoate (HCl salt, 21.0 g, 53.5 mmol) in DMF (200 mL) at 0° C. was added HBTU (30.4 g, 80.3 mmol) and HOBt (10.8 g, 80.3 mmol). The mixture was stirred for 5 min then 2-morpholinoacetic acid (8.15 g, 56.2 mmol) and DIEA (46.5 mL, 214 mmol) were added. The reaction mixture was stirred at ambient temperature for 30 min. Saturated aqueous NaHCO3 (200 mL) was then added and the resulting mixture was extracted with EtOAc (300 mL×2). The combined extracts were washed with brine (400 mL), dried over anhydrous sodium sulfate, and concentrated. Purification of the residue by flash column chromatography on silica gel (heptane to EtOAc/heptane=3:2) afforded (S)-benzyl 3-(4-methoxyphenyl)-2-((S)-2-(2-morpholinoacetamido)propanamido)propanoate (23.0 g, 89% yield) as a colorless solid.

後処理

  1. stirringThe reaction mixture was stirred at ambient temperature for 30 min
  2. extractionthe resulting mixture was extracted with EtOAc (300 mL×2)
  3. washThe combined extracts were washed with brine (400 mL)
  4. dry with materialdried over anhydrous sodium sulfate
  5. concentrationconcentrated
  6. customPurification of the residue by flash column chromatography on silica gel (heptane to EtOAc/heptane=3:2)

出典と根拠

ORDレコード
ord-e9ae184a612a46c3a79556cdcc48bb7f
出典データセット
uspto-grants-2016_09
特許番号
US09434761B2

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