Acetamide → Acetamide Sorbitol

HRID 657443

反応式

HRID 657443 の反応式
[C:1]([NH2:4])(=[O:3])[CH3:2].[Cl-].[Cs+].[O:7]=[CH:8][C@@H:9]([C@H:11]([C@@H:13]([C@@H:15]([CH2:17][OH:18])[OH:16])[OH:14])[OH:12])[OH:10].OP([O-])(O)=O.[K+]>>[OH:18][CH2:17][C@@H:15]([C@H:13]([C@@H:11]([C@@H:9]([CH2:8][OH:7])[OH:10])[OH:12])[OH:14])[OH:16].[C:1]([NH2:4])(=[O:3])[CH3:2]

参加物

5

反応物 4

HCID 178AcetamideHCID 24293Cesium ChlorideHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 516951Monobasic potassium phosphate

生成物 1

HCID 86637219Acetamide Sorbitol

反応条件

温度
55 °C

実験手順

Acetamide (Aldrich 99% sublimed)—0.6 g/L; CsCl—1.68 g/L; Glucose—20 g/L; KH2PO4—20 g/L; MgSO4.7H2O—0.6 g/L; CaCl2.2H2O—0.6 g/L; 1000× salts (see below)—1 ml. pH adjusted to 5.5 and volume brought to 300 ml. Filter sterilized with 0.22 micron filter and warmed to 55° C. in an oven. To 700 ml water Noble Agar (low-melt for top agar) 20 g and Sorbitol 218 g was added and then autoclaved. This mixture was cooled to 55° C., and filter sterilized, acetamide mix was added. Plates or tubes were poured. 1000× Salts—FeSO4.7H2O (0.5 g/100 ml); MnSO4.H2O (0.16 g/100 ml); ZnSO4.7H2O (0.14 g/100 ml); CoCl2.6H2O (0.1 g/100 ml) and filter sterilize with 0.22 micron filter. Potato Dextrose Agar (PDA Difco Dehydrated Culture Media)—Potatoes, infusion from 200 g/L; Dextrose, 20 g/L and Agar, 15 g/L were mixed well in 50-80% final volume of dH2O, and then brought to 100% final volume. This mixture was autoclaved, cooled to 55° C. and poured. To make up 1% skim milk agar for a pH 3.5 media PDA was prepared as above and to 100 ml molten PDA, 1.8 ml 10% tartaric acid and 12.5 ml sterilized 8% skim milk was added and plates were poured. To pre-sterilize skim milk, 8% skim milk (Difco) was autoclaved for 10 minutes, 122-123° C., and chamber pressure during exposure of 32-35 psi. The mixture was removed, cooled and stored at room temperature.

後処理

  1. filtrationFilter
  2. filtrationfilter
  3. additionTo 700 ml water Noble Agar (low-melt for top agar) 20 g and Sorbitol 218 g was added
  4. temperatureThis mixture was cooled to 55° C.
  5. filtrationfilter
  6. additionacetamide mix
  7. additionwas added
  8. additionPlates or tubes were poured
  9. filtrationCoCl2.6H2O (0.1 g/100 ml) and filter sterilize with 0.22 micron
  10. filtrationfilter
  11. additionDextrose, 20 g/L and Agar, 15 g/L were mixed well in 50-80% final volume of dH2O
  12. temperaturecooled to 55° C.
  13. additionpoured
  14. customwas prepared as above and to 100 ml molten PDA, 1.8 ml 10% tartaric acid and 12.5 ml
  15. additionwas added
  16. additionplates were poured
  17. customThe mixture was removed
  18. temperaturecooled
  19. customstored at room temperature

出典と根拠

ORDレコード
ord-c58a32f2144a4506944711bd796773ee
出典データセット
uspto-grants-2011_12
特許番号
US08075694B2

関連ノート

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