Tromethamine hydrochloride → Prostaglandin D2

HRID 6685

反応式

HRID 6685 の反応式
C(O)C(N)(CO)CO.Cl.[Mg+2].[Cl-].[Cl-].[CH3:13][CH2:14][CH2:15][CH2:16][CH2:17][C@H:18]([OH:37])/[CH:19]=[CH:20]/[C@H:21]1[C:25](=[O:26])[CH2:24][C@H:23]([OH:27])[C@@H:22]1[CH2:28]/[CH:29]=[CH:30]/[CH2:31][CH2:32][CH2:33][C:34]([OH:36])=[O:35]>>[CH3:13][CH2:14][CH2:15][CH2:16][CH2:17][C@H:18]([OH:37])/[CH:19]=[CH:20]/[C@H:21]1[C:25](=[O:26])[CH2:24][C@H:23]([OH:27])[C@@H:22]1[CH2:28]/[CH:29]=[CH:30]\[CH2:31][CH2:32][CH2:33][C:34]([OH:36])=[O:35]

参加物

4

反応物 3

HCID 93573Tromethamine hydrochlorideHCID 5360315Magnesium ChlorideHCID 6519333(E)-7-[(1R,2R,5S)-5-hydroxy-2-[(E,3S)-3-hydroxyoct-1-enyl]-3-oxocyclopentyl]hept-5-enoic acid

生成物 1

HCID 448457Prostaglandin D2

実験手順

To a binding-reaction solution (50 mM Tris/HCl, pH 7.4, 5 mM MgCl2) (0.2 ml) were added the human platelet membrane fraction (0.1 mg) and 5 nM [3H]PGD2 (115 Ci/mmol), and the mixture was reacted at 4° C. for 90 min. After the reaction, the mixture was filtered through a glass fiber filter paper and washed several times with cooled physiological saline, then the radioactivity retained on the filter paper was measured. The specific-binding ratio was calculated by subtracting the non-specific binding ratio which is the radioactivity similarly measured in the presence of 10 μM PGD2 from the total binding. The inhibitory activity of each compound was expressed as the concentration required for 50% inhibition (IC50), which was determined by depicting a substitution curve by plotting the binding ratio (%) in the presence of each compound, where the binding ratio in the absence of a test compound is 100%.

後処理

  1. customthe mixture was reacted at 4° C. for 90 min
  2. customAfter the reaction
  3. filtrationthe mixture was filtered through a glass fiber
  4. filtrationfilter paper
  5. washwashed several times
  6. temperaturewith cooled physiological saline
  7. concentrationThe inhibitory activity of each compound was expressed as the concentration

出典と根拠

ORDレコード
ord-3b8414190c404782927a055505deeae4
出典データセット
uspto-grants-2006_08
特許番号
US07084136B2

関連ノート

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