Daidzin → Daidzin

HRID 705060

反応式

HRID 705060 の反応式
[CH:1]1[C:6]([C:7]2[C:16](=[O:17])[C:15]3[CH:14]=[CH:13][C:12]([O:18][C@@H:19]4[O:24][C@H:23]([CH2:25][OH:26])[C@@H:22]([OH:27])[C@H:21]([OH:28])[C@H:20]4[OH:29])=[CH:11][C:10]=3[O:9][CH:8]=2)=[CH:5][CH:4]=[C:3]([OH:30])[CH:2]=1.[CH:31]1[C:36]([C:37]2[C:46](=[O:47])[C:45]3[C:44]([OH:48])=[CH:43][C:42]([O:49][C@@H:50]4[O:55][C@H:54]([CH2:56][OH:57])[C@@H:53]([OH:58])[C@H:52]([OH:59])[C@H:51]4[OH:60])=[CH:41][C:40]=3[O:39][CH:38]=2)=[CH:35][CH:34]=[C:33]([OH:61])[CH:32]=1>C([O-])(=O)C.[Na+]>[CH:5]1[C:6]([C:7]2[C:16](=[O:17])[C:15]3[CH:14]=[CH:13][C:12]([O:18][C@@H:19]4[O:24][C@H:23]([CH2:25][OH:26])[C@@H:22]([OH:27])[C@H:21]([OH:28])[C@H:20]4[OH:29])=[CH:11][C:10]=3[O:9][CH:8]=2)=[CH:1][CH:2]=[C:3]([OH:30])[CH:4]=1.[CH:35]1[C:36]([C:37]2[C:46](=[O:47])[C:45]3[C:44]([OH:48])=[CH:43][C:42]([O:49][C@@H:50]4[O:55][C@H:54]([CH2:56][OH:57])[C@@H:53]([OH:58])[C@H:52]([OH:59])[C@H:51]4[OH:60])=[CH:41][C:40]=3[O:39][CH:38]=2)=[CH:31][CH:32]=[C:33]([OH:61])[CH:34]=1.[CH:5]1[C:6]([C:7]2[C:16](=[O:17])[C:15]3[CH:14]=[CH:13][C:12]([OH:18])=[CH:11][C:10]=3[O:9][CH:8]=2)=[CH:1][CH:2]=[C:3]([OH:30])[CH:4]=1.[CH:1]1[C:6]([C:7]2[C:16](=[O:17])[C:15]3[C:14]([OH:39])=[CH:13][C:12]([OH:18])=[CH:11][C:10]=3[O:9][CH:8]=2)=[CH:5][CH:4]=[C:3]([OH:30])[CH:2]=1

参加物

7

反応物 2

HCID 107971DaidzinHCID 5281377Genistin

生成物 4

HCID 107971DaidzinHCID 5280961GenisteinHCID 5281377GenistinHCID 5281708Daidzein

試薬・触媒・溶媒 1

HCID 517045Sodium Acetate溶媒

実験手順

The digestive juice can be used as-is, or in a purified form. The efficiency of Helix pomatia digestive juice to hydrolyze isoflavone glycosides was established by incubating in vitro 100 μg each of daidzin and genistin with 0.1 mL of Helix pomatia digestive juice suspended in 10 mL of 0.05M sodium acetate buffer, pH 4.5 at 37° C. Before adding the enzyme/buffer mixture, it was passed through a solid-phase C18 Bond Elut cartridge to remove residual amounts of isoflavones that we have previously found to naturally occur in this enzyme preparation. The concentrations of daidzin and genistin remaining, and daidzein and genistein formed during incubation were determined by HPLC on aliquots of the mixture removed at timed intervals over the next 24 hours.

後処理

  1. customat 37° C
  2. additionBefore adding the enzyme/buffer mixture, it
  3. customto remove residual amounts of isoflavones that we
  4. customto naturally occur in this enzyme preparation

出典と根拠

ORDレコード
ord-93e4d88f839d4632a91e2924b97b0f4d
出典データセット
uspto-grants-2015_11
特許番号
US09173866B2

関連ノート

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