Lithium Hydroxide → beta-[[3-[4-(Aminoiminomethyl)benzyl]aminobenzoyl]amino]propanoic acid
反応式
[NH2:1][N:2]=[CH:3][C:4]1[CH:27]=[CH:26][C:7]([CH2:8][NH:9][C:10]2[CH:11]=[C:12]([CH:23]=[CH:24][CH:25]=2)[C:13]([NH:15][CH2:16][CH2:17][C:18]([O:20]CC)=[O:19])=[O:14])=[CH:6][CH:5]=1.[OH-].[Li+]>CO>[NH2:1][N:2]=[CH:3][C:4]1[CH:27]=[CH:26][C:7]([CH2:8][NH:9][C:10]2[CH:11]=[C:12]([CH:23]=[CH:24][CH:25]=2)[C:13]([NH:15][CH2:16][CH2:17][C:18]([OH:20])=[O:19])=[O:14])=[CH:6][CH:5]=1参加物
4
実験手順
Ethyl β-[[3-[4-(aminoiminomethyl)benzyl]aminobenzoyl]amino]propanoate (75 mg) was treated with 1N lithium hydroxide and methanol (1:1; 20 ml) for 5 min. Methanol was removed under reduced pressure and the residue was purified by HPLC using acetonitrile/water/trifluoroacetic acid system. The desired fractions were collected and lyophilized to give 66 mg of white material. FAB-MS: MH+ =341.2.