alpha-Tocopherol → beta-Carotene linoleic

HRID 73884

反応式

HRID 73884 の反応式
[CH3:1][C:2]1[CH2:7][CH2:6][CH2:5][C:4]([CH3:9])([CH3:8])[C:3]=1/[CH:10]=[CH:11]/[C:12](/[CH3:40])=[CH:13]/[CH:14]=[CH:15]/[C:16](/[CH3:39])=[CH:17]/[CH:18]=[CH:19]/[CH:20]=[C:21](\[CH3:38])/[CH:22]=[CH:23]/[CH:24]=[C:25](\[CH3:37])/[CH:26]=[CH:27]/[C:28]1[C:33]([CH3:35])([CH3:34])[CH2:32][CH2:31][CH2:30][C:29]=1[CH3:36].CC1C(O)=C(C)C2CC[C@](CCC[C@@H](CCC[C@@H](CCCC(C)C)C)C)(C)OC=2C=1C.[C:72]([OH:91])(=[O:90])[CH2:73][CH2:74][CH2:75][CH2:76][CH2:77][CH2:78][CH2:79]/[CH:80]=[CH:81]\[CH2:82]/[CH:83]=[CH:84]\[CH2:85][CH2:86][CH2:87][CH2:88][CH3:89]>>[CH3:36][C:29]1[CH2:30][CH2:31][CH2:32][C:33]([CH3:34])([CH3:35])[C:28]=1/[CH:27]=[CH:26]/[C:25](/[CH3:37])=[CH:24]/[CH:23]=[CH:22]/[C:21](/[CH3:38])=[CH:20]/[CH:19]=[CH:18]/[CH:17]=[C:16](\[CH3:39])/[CH:15]=[CH:14]/[CH:13]=[C:12](\[CH3:40])/[CH:11]=[CH:10]/[C:3]1[C:4]([CH3:9])([CH3:8])[CH2:5][CH2:6][CH2:7][C:2]=1[CH3:1].[CH3:89][CH2:88][CH2:87][CH2:86][CH2:85]/[CH:84]=[CH:83]\[CH2:82]/[CH:81]=[CH:80]\[CH2:79][CH2:78][CH2:77][CH2:76][CH2:75][CH2:74][CH2:73][C:72]([OH:91])=[O:90]

参加物

4

反応物 3

HCID 14985alpha-TocopherolHCID 5280450Linoleic AcidHCID 5280489Beta-Carotene2 回

生成物 1

HCID 87661994beta-Carotene linoleic

実験手順

Beta-carotene bleaching (BCB) assay for kenaf seed and commercial edible oils were determined according to the protocol described by Kumazawa et al. (2002). α-tocopherol was used as the standard lipophilic antioxidant in this test. In brief, 3 ml of beta-carotene solution (100 μg/ml chloroform) (Sigma-Aldrich Co., St. Louis, Mo., USA) was added to 40 mg of linoleic acid (Sigma-Aldrich Co., St. Louis, Mo., USA) and 400 mg of Tween 20 (Fisher Scientific, Loughborough, Leicestershire, UK). Then, the mixture was mixed well and dried under nitrogen stream. Immediately, 100 ml of distilled water was added to the dried mixture to form a beta-carotene/linoleic emulsion. One and half milliliters of emulsion were added with 20 μl of methanol (control) or sample at the concentration of 5 mg/ml methanol. After that, the sample was incubated in water bath at 50° C. for one hour. Finally, the absorbance of the sample was measured at 470 nm against distilled water (blank) by using a UV-Visible spectrophotometer (Pharmaspec uv-1700, Shimadzu, Kyoto, Japan).

後処理

  1. additionThen, the mixture was mixed well
  2. customdried under nitrogen stream
  3. additionImmediately, 100 ml of distilled water was added to the dried mixture

出典と根拠

ORDレコード
ord-ddcd35c464a64bf19859c50217fe308b
出典データセット
uspto-grants-2014_09
特許番号
US08829214B2

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