反応式
C[C@]1(O)[C@@H]2C(=[C:12]([OH:30])[C@:13]3([OH:29])[C:20](=[O:21])[C:19](C(N)=O)=[C:18]([OH:25])[C@@H:17](N(C)C)[C@@H]3C2)C(=O)C2C(O)=CC=CC1=2.CC1(C)S[C@@H]2[C@H](NC([C@H](N)C3C=CC=CC=3)=O)C(=[O:41])N2[C@H]1C(O)=O>>[O:30]=[CH:12][C@@H:13]([C@@H:20]([C@H:19]([C@H:18]([CH3:17])[OH:25])[OH:41])[OH:21])[OH:29]参加物
3
実験手順
The plasmid pBRsacH3 (Example 1) was used for cloning the rMET1 antibody (Example 9). In this case, the kappa and heavy chains were engineered with human ceruloplasmin and human neutrophil defensin signal sequences, respectively (Example 9). This plasmid was electroporated into E. coli strain DH10B rha+ to generate clone rMET1.LS.1, which was cultured under 10 μg/mL tetracycline selection. The plasmid was also electroporated into E. coli strain ECL339 rha− to generate clone rMET1.ECL.1 which was cultured under 100 μg/mL ampicillin selection.
出典と根拠
- ORDレコード
- ord-d4993eb2a11a4ed2b1d918772a04add8
- 出典データセット
- uspto-grants-2008_07
- 特許番号
- US07396661B2
関連ノート
読み込み中…