2-Oxoglutaric Acid → cis-3-Hydroxy-L-proline

HRID 848959

反応式

HRID 848959 の反応式
[NH:1]1[CH2:8][CH2:7][CH2:6][C@H:2]1[C:3]([OH:5])=[O:4].[O:9]=C(CCC(O)=O)C(O)=O.O=C1O[C@H]([C@H](CO)O)C(O)=C1O>>[OH:9][C@@H:6]1[CH2:7][CH2:8][NH:1][C@@H:2]1[C:3]([OH:5])=[O:4]

参加物

4

反応物 3

HCID 512-Oxoglutaric AcidHCID 145742L-ProlineHCID 54670067L-Ascorbic Acid

生成物 1

HCID 7098652cis-3-Hydroxy-L-proline

反応条件

温度
100 °C

実験手順

The enzyme preparation to be determined is added to 100 mM TES buffer (pH 7.0) containing 5 mM L-proline, 5 mM 2-ketoglutaric acid, 1 mM ferrous sulfate and 5 mM L-ascorbic acid to make 100 μl in total, and the mixture was allowed to stand at 35° C. for 10 minutes. The reaction mixture is heated at 100° C. for 2 minutes so as to stop the reaction, and the amount of cis-3-hydroxy-L-proline produced in the reaction mixture is determined by high performance liquid chromatography (hereinafter referred to as HPLC).

後処理

  1. customThe enzyme preparation
  2. additionto be determined is added to 100 mM TES buffer (pH 7.0)
  3. customthe reaction

出典と根拠

ORDレコード
ord-403476d414564f0992b50f896ab7cf8a
出典データセット
uspto-grants-2000_01
特許番号
US06010891

関連ノート

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