Water → {1-[4-(7-Amino-3-phenyl-imidazo[1,2-a]pyridin-2-yl)-phenyl]-cyclobutyl}-carbamic acid tert-butyl ester

温度

100 °C

A solution of 2-[4-(1-tert-butoxycarbonylamino-cyclobutyl)-phenyl]-3-phenyl-imidazo[1,2-a]pyridine-7-carboxylic acid (1.20 g, 0.86 mmol) and 10% palladium on carbon (0.2 g) in MeOH (20 mL) in DMF (40 mL) and water (3.4 mL) was added triethylamine (0.38 mL, 2.73 mmol, 1.1 equiv) followed by diphenylphosphoryl azide (751 mg, 2.73 mmol, 1.1 equiv). The resulting mixture was stirred at room temperature 10 minutes, at 100° C. for 6 h, at room temperature for 12 h. To the resulting mixture was added triethylamine (0.38 mL, 2.73 mmol, 1.1 equiv) followed by diphenylphosphoryl azide (751 mg, 2.73 mmol, 1.1 equiv). The resulting mixture was heated at 100° C. for 12 h. The resulting mixture was separated between EtOAc (100 mL) and water (50 mL). The organic phase was dried (Na2SO4 anh) and concentrated under reduced pressure. The remaining material (1.4 g) was purified using MPLC (Biotage Isolera Flash NH2 Snap 10 reverse phase column; 100% CH2Cl2 for 3.5 min., gradient to 90% CH2Cl2: 10% MeOH over 1 min.; 90% CH2Cl2: 10% MeOH for 5.5 min., gradient to 80% CH2Cl2: 20% MeOH over 6 min., 80% CH2Cl2: 20% MeOH for 10.2 min.) to give {1-[4-(7-amino-3-phenyl-imidazo[1,2-a]pyridin-2-yl)-phenyl]-cyclobutyl}-carbamic acid tert-butyl ester (508 mg, 45%):

1. temperatureThe resulting mixture was heated at 100° C. for 12 h
2. customThe resulting mixture was separated between EtOAc (100 mL) and water (50 mL)
3. dry with materialThe organic phase was dried (Na2SO4 anh)
4. concentrationconcentrated under reduced pressure
5. customThe remaining material (1.4 g) was purified
6. wait100% CH2Cl2 for 3.5 min.
7. waitgradient to 90% CH2Cl2: 10% MeOH over 1 min.
8. wait90% CH2Cl2: 10% MeOH for 5.5 min.
9. waitgradient to 80% CH2Cl2: 20% MeOH over 6 min.

ORDレコード

ord-1e2173c622eb48d0a6fb21cdc5044da7

出典データセット

uspto-grants-2015_03

DOI

10.6084/m9.figshare.5104873.v1

特許番号

US08975265B2