HCID 178448348、化合物 → HCID 178476271、化合物
反応式
[NH2:1][C@H:2]([C:10]([NH:12][C@H:13]([C:26]([NH:28][C@H:29]([C:39]([NH:41][C@H:42]([C:46]([NH:48][C@H:49]([C:62]([O:64]C(C)(C)C)=[O:63])[CH2:50][C:51]1[CH:56]=[CH:55][C:54]([O:57]C(C)(C)C)=[CH:53][CH:52]=1)=[O:47])[CH:43]([CH3:45])[CH3:44])=[O:40])[CH2:30][CH2:31][C:32](=[O:38])[O:33]C(C)(C)C)=[O:27])[CH2:14][CH2:15][CH2:16][CH2:17][NH:18]C(OC(C)(C)C)=O)=[O:11])[CH2:3][CH2:4][CH2:5][NH:6][C:7](=[NH:9])[NH2:8].Cl.Cl>FC(F)(F)C(O)=O.C(S)C>[NH2:1][C@H:2]([C:10]([NH:12][C@H:13]([C:26]([NH:28][C@H:29]([C:39]([NH:41][C@H:42]([C:46]([NH:48][C@H:49]([C:62]([OH:64])=[O:63])[CH2:50][C:51]1[CH:56]=[CH:55][C:54]([OH:57])=[CH:53][CH:52]=1)=[O:47])[CH:43]([CH3:44])[CH3:45])=[O:40])[CH2:30][CH2:31][C:32](=[O:33])[OH:38])=[O:27])[CH2:14][CH2:15][CH2:16][CH2:17][NH2:18])=[O:11])[CH2:3][CH2:4][CH2:5][NH:6][C:7](=[NH:8])[NH2:9].[CH3:31][C:32]([OH:38])=[O:33]参加物
4
実験手順
1 g of H-Arg-Lys(Boc)-Glu(OBut)-Val-Tyr(But)-OBut.2HCl (0.966 mmole) is dissolved in a mixture of 10 ml of trifluoroacetic acid and 1 ml of ethylmercaptan. The mixture is allowed to stand for one hour at room temperature and is concentrated. The residue is dissolved again in a little methanol and the solution is concentrated once more. The residue is dissolved in water, insoluble matter is filtered off and the solution is chromatographed over a strongly basic exchanger in the acetate form. The eluate is concentrated and purified further by being chromatographed on a hydroxypropylated crosslinked dextran gel in a water/acetic acid/n-butanol mixture. Yield 491 mg (57%), [α]D =-21.8° (c=1, water). Aminoacid analysis: Glu 1.00, Val 0.97, Tyr 0.92, Lys 1.03 and Arg 0.99. Protein content: 82%.