Methanol → 2,2'-Anhydro-1-beta-D-arabinofuranosylcytosine hydrochloride

A 500 mL flask was charged with 50 mL methanol, 1.95 g cytidine and 2 g dibutyl tin oxide. The resulting suspension was refluxed for five hours and then stirred at room temperature for twelve hours. To the mixture was then added triethylamine (7.8 mL) followed by slow addition of p-toluenesulfonyl chloride (10.68 g). The resulting mixture was stirred for twelve hours at room temperature. Thereafter, the solvents were evaporated under vacuum and chloroform (100 mL) was added to the resulting white gum. The chloroform/white gum suspension was refluxed for fifteen minutes and then cooled to room temperature. The resulting white precipitate was filtered and washed with chloroform, and dried to yield 1 g of crude 2,2'-O-cyclocytidine hydrochloride. The crude cyclocytidine hydrochloride was suspended in 5 mL water and the mixture was heated to 60° C. This solution was filtered and the solvent reduced under vacuum to obtain a turbid oil. Ethanol (18 mL) was added and the mixture was stirred at 5° C. for twelve hours. The resulting precipitate was filtered and dried to provide 0.6 g of pure 2,2'-O-cyclocytidine hydrochloride (29% yield). The product was characterized by comparison of its melting point, and NMR and IR spectra with those previously reported for 2,2'-O-cyclocytidine.

1. temperatureThe resulting suspension was refluxed for five hours
2. stirringThe resulting mixture was stirred for twelve hours at room temperature
3. customThereafter, the solvents were evaporated under vacuum and chloroform (100 mL)
4. additionwas added to the resulting white gum
5. temperatureThe chloroform/white gum suspension was refluxed for fifteen minutes
6. temperaturecooled to room temperature
7. filtrationThe resulting white precipitate was filtered
8. washwashed with chloroform
9. customdried

ORDレコード

ord-a2a1099b2be442b1ac6ed3beb73fda27

出典データセット

uspto-grants-1997_03

DOI

10.6084/m9.figshare.5104873.v1

特許番号

US05610292