5-Fluorouridine → Floxuridine

5.244 gms. (20 mMoles) of 5-fluorouridine is dissolved in 20 mL anhydrous pyridine and then dried under vacuum. This procedure is repeated at least one more time in order to make 5-fluorouridine devoid of moisture. The dried 5-fluorouridine is then dissolved in 20 mL of pyridine. 8.131 gms (24 mMoles, 1.2 eq.) of 4,4'-dimethoxy trityl chloride is added as solid in 4 fractions over a period of 2 hours. The reaction mixture is then stirred at room temperature overnight and is then diluted with 25 mL of dichloromethane and washed with saturated sodium bicarbonate solution. The aqueous layer is then washed with 20 mL dichloromethane. The organic layers are mixed and washed with saturated bicarbonate solution two more times. The organic layer is then dried over anhydrous sodium sulphate. The solvent is removed under vacuum to yield a yellow foam which is purified on a silica gel column using 90:10 chloroform:methanol as eluent. The 4, 4'-dimethoxytrityl derivative of 5-fluorodeoxyuridine is prepared in an analogous manner.

1. customdried under vacuum
2. dissolutionThe dried 5-fluorouridine is then dissolved in 20 mL of pyridine
3. washwashed with saturated sodium bicarbonate solution
4. washThe aqueous layer is then washed with 20 mL dichloromethane
5. additionThe organic layers are mixed
6. washwashed with saturated bicarbonate solution two more times
7. dry with materialThe organic layer is then dried over anhydrous sodium sulphate
8. customThe solvent is removed under vacuum
9. customto yield a yellow foam which
10. customis purified on a silica gel column

ORDレコード

ord-eaea4fec596542cfb14946f45a1e0e5b

出典データセット

uspto-grants-1997_03

DOI

10.6084/m9.figshare.5104873.v1

特許番号

US05614505