Fluorescein 5-isothiocyanate
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기본 정보
- CAS
3326-32-7- 표준 SMILES
O=C1OC2(c3ccc(O)cc3Oc3cc(O)ccc32)c2ccc(N=C=S)cc21- InChIKey
MHMNJMPURVTYEJ-UHFFFAOYSA-N- 분자식
- C21H11NO5S
- 평균 분자량
- 389.4 g/mol
- 단동위원소 질량
- 389.03579362
개요
Fluorescein 5-isothiocyanate is the 5-isomer of fluorescein isothiocyanate. Acts as a fluorescent probe capable of being conjugated to tissue and proteins; used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.
이름 및 식별자
- 우선 이름
- Fluorescein 5-isothiocyanate
- IUPAC
- 3',6'-dihydroxy-6-isothiocyanatospiro[2-benzofuran-3,9'-xanthene]-1-one
- 영어명
- Fluorescein 5-isothiocyanate
- 분자식
- C21H11NO5S
- 분자량
- 389.38
이름 및 동의어
3326-32-75-FITCFLUORESCEIN-5-ISOTHIOCYANATEfluorescein 5-isothiocyanate5-isothiocyanatofluoresceinSpiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-5-isothiocyanato-CHEBI:37918DTXSID80892440I223NX31W9Fluorescein 5 isothiocyanate HydrochlorideHydrochloride, Fluorescein-5-isothiocyanateSpiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 3',6'-dihydroxy-5-isothiocyanato-
이름 더 보기
Fluorescein isothiocyanate isomer5-FLUORESCEIN ISOTHIOCYANATEFluorescein isothiocyanate isomer I, tech gradeFITC-IFluorescein isothiocyanate IFluorescein isothiocyanate,Isomer I(FITC)
5 IsothiocyanatofluoresceinFluorescein 5 isothiocyanateRefChem:140896DTXCID3029368222-042-0FITCFluorescein isothiocyanate isomer I3',6'-dihydroxy-5-isothiocyanato-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one
데이터베이스 식별자
물성
구조 계산 물성보충 중
- XLogP
- 4.8
- 극성 표면적
- 120 Ų
- 수소 결합 공여자 수
- 2
- 수소 결합 수용자 수
- 7
- 회전 가능 결합 수
- 1
- 무거운 원자 수
- 28
- 형식 전하
- 0
- 복잡도
- 668
실험 및 물리화학적 성질
Chemical ClassesDyes -> Indicator Dyes
Physical DescriptionOrange solid; [Merck Index] Orange powder; [Alfa Aesar MSDS]
물리화학적 성질
- 녹는점
- 360 °C · >360 °C(lit.)
- 끓는 점
- 708.6 · 708.6±60.0 °C(Predicted)
- 밀도
- 1.3423 · 1.3423 (rough estimate)
- 굴절률
- 1.506 · 1.5060 (estimate)
- 저장 조건
- 2 · 2-8°C
- 용해도
- DMSO, 에탄올, 메탄올 DMF 및 아세톤에 약간 용해됨
- 물리적 상태
- 가루
- 산도 계수 (pKa)
- 2.2 · 2.2, 4.4, 6.7(at 25℃)
- 색상
- 하얀색
- 수용성
- 실질적으로 불용성
- BRN
- 1407295 · 1407295
- 안정성
- 안정성 안정적이지만 습기에 민감합니다. 강한 산화제, 강염기, 아민, 산과 호환되지 않습니다.
- 주요 응용
- 자기 나노와이어, 면역 분석법, 단백질 분석, 염색체 식별, 살모넬라균, 암 진단, 신장 질환, 병원균 검출, 유전자, 살모넬라 세포, 톡소플라스마증, 효소 매개 핵산 분해, 대장암 표면 분자, 암 치료, 만성 림프구성 백혈병
안전 및 규제
안전 정보는 참고용이며, 공급업체의 물질안전보건자료(SDS), 관련 법규 및 전문가 평가를 대체하지 않습니다.
GHS 분류
GHS Classification
Danger
H317 (100%): May cause an allergic skin reaction [Warning Sensitization, Skin]
P233, P260, P261, P271, P272, P280, P284, P302+P352, P304+P340, P321, P333+P317, P342+P316, P362+P364, P403, and P501 (click each P-code to see the statement)
Aggregated GHS information provided per 169 reports by companies from 6 notifications to the ECHA C&L Inventory. Each notification may be associated with multiple companies.
유해성 정보
Regulatory Information
Chemical: Spiro[isobenzofuran-1(3H),9'-(9H)xanthen]-3-one, 3',6'-dihydroxy-5-isothiocyanato-
Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-5-isothiocyanato-: Does not have an individual approval but may be used under an appropriate group standard
Hazards SummaryCauses cutaneous sensitization (experimental) and effects on inflammation in 3-day intermittent dermal studies of mice; [RTECS] May cause irritation; May cause skin and respiratory sensitization; [Alfa Aesar MSDS]
Hazard Classes and CategoriesSkin Sens. 1 (100%)
독성 정보
Treatment
EYES: irrigate opened eyes for several minutes under running water. INGESTION: do not induce vomiting. Rinse mouth with water (never give anything by mouth to an unconscious person). Seek immediate medical advice. SKIN: should be treated immediately by rinsing the affected parts in cold running water for at least 15 minutes, followed by thorough washing with soap and water. If necessary, the person should shower and change contaminated clothing and shoes, and then must seek medical attention. INHALATION: supply fresh air. If required provide artificial respiration.
화학 및 산업
약리 정보
Metabolism/Metabolites
Cyanide is rapidly alsorbed through oral, inhalation, and dermal routes and distributed throughout the body. Cyanide is mainly metabolized into thiocyanate by either rhodanese or 3-mercaptopyruvate sulfur transferase. Cyanide metabolites are excreted in the urine. (L96)
MeSH Pharmacological Classification
Chemicals that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
용도 및 제조
Uses
Used as a fluorescent indicator to probe biological systems and monitor sewage; [Merck Index]
Fluorescein isothiocyanate is used in flow cytometry. (L552)
General Manufacturing InformationSpiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-5-isothiocyanato-: ACTIVE
응용 분야
개요Fluorescein 5-isothiocyanate (5-FITC) is an isomer of fluorescein isothiocyanate (FITC), which is commonly used as a mixture of the 5- and 6-isothiocyanate isomers. It reacts with amine and thiol groups to form conjugates with proteins, lipids, and other molecules for detection by a variety of fluorescent-based applications. 5-FITC displays excitation/emission maxima of 494/519 nm, respectively.
일반 설명Fluorescein isothiocyanate (FITC) is yellow-orange in color with an absorption maximum at 495nm. Upon excitation, it emits a yellow-green color with an emission maximum at 525nm.It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.Fluorescein isothiocyanate isomer I has been proposed as a contact sensitizer.
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