Sodium Carbonate → 2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid
반응식
C(=O)([O-])[O-].[Na+].[Na+].[CH:7]1[C:12](N=C=S)=[CH:11][C:10]2[C:16]([O:18][C:19]3([C:29]4[CH:30]=[CH:31][C:32]([OH:34])=[CH:33][C:28]=4[O:27][C:21]4[CH:22]=[C:23]([OH:26])[CH:24]=[CH:25][C:20]3=4)[C:9]=2[CH:8]=1)=[O:17]>CN(C)C=O>[CH:7]1[CH:12]=[CH:11][C:10]([C:16]([OH:18])=[O:17])=[C:9]([C:19]2[C:20]3[CH:25]=[CH:24][C:23]([OH:26])=[CH:22][C:21]=3[O:27][C:28]3[C:29]=2[CH:30]=[CH:31][C:32]([CH:33]=3)=[O:34])[CH:8]=1참가 물질
4
실험 절차
Two ml of Fab solution were brought to pH 9.0 by the addition of solid sodium carbonate (Na2CO3). A solution of 1.5 mg of fluorescein isothiocyanate (FITC) in 0.1 ml dry dimethylformamide (DMF) was slowly added (over 1/2 min.) with stirring at room temperature. After an additional 2 hrs., the solution was briefly clarified on a Brinkmann centrifuge (21/2 min.) and applied for separation into a column of Sephadex G-25 (0.9×15 cm) equilibrated with 0.05 M KPO4 buffer at pH 8.0. The yellow protein band (first band eluted) was collected. The final solution was 10.4 mg/ml in protein and degree of labeling 4.1 fluoresceins per Fab.