트리스(하이드록시메틸)아미노메탄 수화염화물

TRIS hydrochloride is a white crystalline powder that has a pKa of 8.08 at 25°C and pH range of 7.0 to 9.0 that coincides with the physiological pH of most living organisms because of which it is commonly used as a component of buffer solutions in biology, biochemistry and molecular biology applications. Tris salts are also used for crystallization of proteins at various pH values. Neither Tris base nor Tris hydrochloride by itself provide adequate buffering capacity. Generally the two need to be mixed together to provide a buffer with pH between 7 and 9 to provide adequate buffering.

개요Abbreviated as Tris HCl (TRIS hydrochloride), Tris (hydroxymethyl) aminomethane hydrochloride is an organic compound commonly utilized in buffer solutions such as TAE or TBE for electrophoresis gels. It boasts high solubility in water and offers utility within the pH range of 7.0-9.0.

용도Tris Hydrochloride is a buffering medium for electrophoresis, molecular biology, and cell culture applications. It may be combined with Tris Base to simplify Tris buffer preparation. Tris Hydrochloride has been incorporated in coelomic fluid and in Cortland medium to store unfertilized rainbow trout eggs. In immunohistochemistry, Tris Hydrochloride has also been used with urea as a method for antigen retrieval.

일반 설명TRIS hydrochloride is mainly required for preparation of buffer at physiological range of 7.3 to 7.5. The prepared buffer is compatible with biological fluids. It is of importance to laboratories as a standard pH solution.

The preparation of TRIS hydrochloride is as follows:After reacting 2-amino-1,3-propanediol (2.50 g, 27.44 mmol) and formic acid (1.26 g, 27.44 mmol) in 50 ml of water at room temperature for 3 hours, water was distilled off under reduced pressure, A liquid was obtained. By washing the obtained liquid,An ammonium formate salt (3.76 g, 27.44 mmol) was obtained as a colorless transparent liquid.