L-Glutamine → Ethylenediaminetetraacetic Acid

HRID 1013859

반응식

HRID 1013859 반응식
[NH2:1][C@H:2]([C:8]([OH:10])=[O:9])CCC(=O)N.[C:11](=[O:14])([OH:13])[O-].[Na+].[C:16]([O-:21])(=[O:20])[C:17](C)=O.[Na+].CCN(CCOC(C1C=CC(N)=CC=1)=O)CC.CC1(C)S[C@@H:44]2[C@H:46](NC(CC3C=CC=CC=3)=O)[C:47](=O)[N:43]2[C@H:42]1[C:59]([OH:61])=[O:60].O.C[C@@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](NC(N)=N)[C@@H](O)[C@@H]2NC(N)=N)[C@H](O[C@@H]2O[C@@H](CO)[C@H](O)[C@@H](O)[C@@H]2NC)[C@@]1(O)C=O>>[CH2:46]([N:1]([CH2:2][C:8]([OH:10])=[O:9])[CH2:17][C:16]([OH:21])=[O:20])[CH2:44][N:43]([CH2:47][C:11]([OH:13])=[O:14])[CH2:42][C:59]([OH:61])=[O:60]

참가 물질

6

반응물 5

HCID 5961L-Glutamine2회HCID 225024-Thia-1-azabicyclo(3.2.0)heptane-2-carboxylic acid, 3,3-dimethyl-7-oxo-6-((phenylacetyl)amino)-(2S,5R,6R)-, compd. with 2-(diethylamino)ethyl 4-aminobenzoate, hydrate (1:1:1)2회HCID 516892Sodium Bicarbonate2회HCID 23662274Sodium pyruvateHCID 178484872HCID 178484872, 화합물2회

생성물 1

HCID 6049Ethylenediaminetetraacetic Acid

실험 절차

293 cells (human transformed embryonic kidney cell line; ATCC cat#: CRL-1573) were cultivated in DMEM containing 2 mM L-glutamine, 0.1 mM non-essential amino acids, 1.5 g/l sodium bicarbonate, 1 mM sodium pyruvate, 10% FBS, penicillin 100 U/mL and streptomycin 100 microg/mL. A549 (Human lung carcinoma cell lines; ATCC) were cultivated in Ham's F12 media containing 2 mM L-glutamine; 1.5 g/L sodium bicarbonate; 10% FBS, Penicillin 100 U/mL, Streptomycin 100 microg/mL. Human peripheral blood mononuclear cells (PBMC) (peripheral blood mononuclear cells) were obtained from healthy volunteers. PBMC were isolated by Ficoll-Hypaque density gradient centrifugation of EDTA K3 blood from normal donors. PBMC were plated at 2×106 cells/mL/well in 12 well plates in AIM-V culture media supplemented with Penicillin 100 U/mL, Streptomycin 100 microg/mL. CYNOM-K1 cells were cultivated in EMEM containing 2 mM L-glutamine, 1% non-essential amino acids, 10% FBS, penicillin 100 U/mL and streptomycin 100 microg/mL. NHBE cells were cultivated in BEBM (500 mL supplemented with BPE, 2 ml; Hydrocortisone, 0.5 ml; hEGF, 0.5 ml; Epinephrine, 0.5 ml; Transferrin, 0.5 ml; Insulin, 0.5 ml; Retinoic Acid, 0.5 ml; Triiodothyronine, 0.05 ml).

후처리

  1. customHuman peripheral blood mononuclear cells (PBMC) (peripheral blood mononuclear cells) were obtained from healthy volunteers

출처 및 근거

ORD 기록
ord-00f6c98cec094997b3f2c838cb356024
출처 데이터셋
uspto-grants-2011_07
특허 번호
US07982028B2

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