Urea → 2-keto-L-Gulonic acid

HRID 1033722

반응식

HRID 1033722 반응식
[OH:1][CH2:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].NC(N)=[O:15].OP([O-])(O)=O.[K+].C([O-])([O-])=O.[Ca+2]>>[CH2:2]([OH:1])[C@H:3]([OH:4])[C@@H:5]([OH:6])[C@H:7]([OH:8])[C:9]([C:11]([OH:15])=[O:12])=[O:10]

참가 물질

5

반응물 4

HCID 1176UreaHCID 5780SorbitolHCID 10112Calcium CarbonateHCID 516951Monobasic potassium phosphate

생성물 1

HCID 1024242-keto-L-Gulonic acid

실험 절차

Five ml of the resulting individual seed cultures as prepared above was inoculated into a 500 ml of Erlenmeyer flask containing 50 ml of production medium (PM): D-sorbitol 8%, corn steep liquor 1%, urea 1.5% (sterilized separately), KH2PO4 0.1%, MgSO4.7H2O 0.01%, CaCO3 0.6% and antifoam 0.1% (pH 7.0 before sterilization) and incubated at 30° C. for 4 days with shaking (180 rpm). Table 1 summarizes the results of the quantitative determination of 2-keto-L-gulonic acid of the cultured broths as assayed by high performance liquid chromatography.

후처리

  1. customFive ml of the resulting individual seed cultures as prepared
  2. additioncontaining 50 ml of production medium (PM)

출처 및 근거

ORD 기록
ord-5749d793ee5347d998bda00a14d9639e
출처 데이터셋
uspto-grants-1994_05
특허 번호
US05312741

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