Diisopropylethylamine → (2S,4R)-methyl 1-((S)-2-((hept-6-enyloxy)carbonylamino)-3,3-dimethylbutanoyl)-4-methoxy-4-(2'-vinylbiphenyl-4-yl)pyrrolidine-2-carboxylate

HATU (248 mg, 0.652 mmol) was added to a solution of (2S,4R)-methyl 4-methoxy-4-(2′-vinylbiphenyl-4-yl)pyrrolidine-2-carboxylate (200 mg, 0.593 mmol), (S)-2-((hept-6-enyloxy)carbonylamino)-3,3-dimethylbutanoic acid (201 mg, 0.741 mmol) and DIEA (0.311 mL, 1.778 mmol) in DCM (8 mL) and stirred at r.t. 60 hours. The reaction was evaporated and purified on the Biotage (10-40% EtOAc:Hex) to give (2S,4R)-methyl 1-((S)-2-((hept-6-enyloxy)carbonylamino)-3,3-dimethylbutanoyl)-4-methoxy-4-(2′-vinylbiphenyl-4-yl)pyrrolidine-2-carboxylate (260 mg, 0.440 mmol, 74% yield) as a white solid. 1H NMR (400 MHz, CHLOROFORM-d) δ ppm 0.96-1.00 (m, 1H) 1.06-1.10 (m, 1H) 1.11-1.19 (m, 7H) 1.30-1.47 (m, 4H) 1.55-1.64 (m, 2H) 1.99-2.10 (m, 2H) 2.49-2.55 (m, 1H) 2.89 (d, J=13.05 Hz, 1H) 2.93-3.04 (m, 3H) 3.73-3.80 (m, 3H) 3.92-4.10 (m, 3H) 4.11-4.20 (m, 1H) 4.31-4.41 (m, 1H) 4.87-5.05 (m, 3H) 5.23 (dd, J=10.92, 1.13 Hz, 1H) 5.38-5.42 (m, 1H) 5.68-5.75 (m, 1H) 5.75-5.86 (m, 1H) 6.62-6.76 (m, 1H) 7.28-7.43 (m, 7H) 7.63-7.69 (m, 1H). LCMS: r.t.=2.25 min., [M+H]+=591 Phenomenex Luna S10 (3×50 mm); Solvent A=95% water—5% methanol—10 mM ammonium acetate, Solvent B=5% water—95% methanol—10 mM ammonium acetate; gradient=0% to 100% solvent B over 2 min. and then hold for 1 min.; 4 ml/min; inj. vol.=10 ul; wavelength=220 nm.

1. custom60 hours
2. customThe reaction was evaporated
3. custompurified on the Biotage (10-40% EtOAc:Hex)

ORD 기록

ord-d4f6c731cea44368941de8071dd0321c

출처 데이터셋

uspto-grants-2013_10

DOI

10.6084/m9.figshare.5104873.v1

특허 번호

US08563505B2