Tyramine → Biotin tyramine

HRID 1080603

반응식

C1S[C@@H](CCCCC(NCCCC[C@H](N)C(O)=O)=O)[C@H]2NC(N[C@@H]12)=O.[NH2:26][CH2:27][CH2:28][C:29]1[CH:34]=[CH:33][C:32]([OH:35])=[CH:31][CH:30]=1.C1C(=O)N([O:43][C:44]([CH2:46][CH2:47][CH2:48][CH2:49][CH:50]2[S:54][CH2:53][CH:52]3[NH:55][C:56]([NH:58][CH:51]23)=[O:57])=[O:45])C(=O)C1>>[OH:45][C:44]([CH2:46][CH2:47][CH2:48][CH2:49][C@H:50]1[C@@H:51]2[C@@H:52]([NH:55][C:56]([NH:58]2)=[O:57])[CH2:53][S:54]1)=[O:43].[NH2:26][CH2:27][CH2:28][C:29]1[CH:34]=[CH:33][C:32]([OH:35])=[CH:31][CH:30]=1

참가 물질

반응물 3

HCID 5610Tyramine

HCID 83814Biocytin

HCID 434213NHS-Biotin

생성물 1

HCID 86746652Biotin tyramine

실험 절차

Para-hydroxyphenylpropionyl biocytin (HPPB) was prepared by mixing a solution of p-hydroxyphenylpropionic acid-N-hydroxysuccinimide ester (50 mg [0.2 mMol]/2 ml dimethyl sulfoxide) with biocytin (70.75 mg [0.2 mMol]/2 ml 0.1M NaHCO3) overnight at room temperature (RT). Biotin-tyramine (BT) was prepared by mixing a solution of tyramine (40 mg [0.3 mMol]/1 ml dimethyl sulfoxide) with biotin-N-hydroxysuccinimide ester (100 mg [0.3 mMol]/1 ml dimethyl sulfoxide) overnight at RT. The solutions of HPPB and BT were used as is. The calculated concentrations were 26 mg/ml for HPPB and 55 mg/ml for BT.

후처리

concentrationThe calculated concentrations

출처 및 근거

ORD 기록: ord-eb10b5356f1040039f7afd213056b076
출처 데이터셋: uspto-grants-1996_12
DOI: 10.6084/m9.figshare.5104873.v1
특허 번호: US05583001

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