반응식
[CH:1]1[CH:6]=[CH:5][C:4]([CH2:7][CH:8]2[NH:14][C:12](=[O:13])[NH:11][C:9]2=[O:10])=[CH:3][CH:2]=1.[OH:15]P(O)(O)=O>>[C:12]([NH:14][C@H:8]([C:9]([OH:15])=[O:10])[CH2:7][C:4]1[CH:5]=[CH:6][CH:1]=[CH:2][CH:3]=1)(=[O:13])[NH2:11]참가 물질
3
실험 절차
The assay of the D-hydantoinase activity was conducted by incubating a reaction mixture containing 120 mg/dl D-5-benzylhydantoin (BH), 50 mM KPB (pH 8.0) and an enzyme solution at 37° C. for 30 minutes, adding 9 volumes of 1.1 mMCuSO4, 11.1 mM H3PO4, centrifuging at 20,000G for 10 minutes to remove the pellet, and then quantifying the resultant N-carbamylphenylalanine (N—Car—Phe) by HPLC. One unit of the enzyme activity was defined as an enzymatic activity capable of producing 1 μmol of N-carbamylphenylalanine per 1 minute under the condition described above.
후처리
- customto remove the pellet
출처 및 근거
- ORD 기록
- ord-0600565ad9de48fdad4dbd63e40730ff
- 출처 데이터셋
- uspto-grants-2006_08
- 특허 번호
- US07098019B2
연결된 노트
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