Glutaraldehyde → Glucose galactose
반응식
[OH:1][CH:2]1[O:21][C@H:20]([CH2:22][OH:23])[C@@H:7]([O:8][C@@H]2O[C@H](CO)[C@H](O)[C@H](O)[C@H]2O)[C@H:5]([OH:6])[C@H:3]1[OH:4].C(=O)CCCC=O>P([O-])([O-])([O-])=O>[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:20]([CH2:22][OH:23])[OH:21])[OH:8])[OH:6])[OH:4].[O:1]=[CH:2][C@@H:3]([C@H:5]([C@H:7]([C@@H:20]([CH2:22][OH:23])[OH:21])[OH:8])[OH:6])[OH:4]참가 물질
4
반응 조건
- 온도
- 60 °C
실험 절차
Pure lactose, demineralised whey and skimmed milk are used as lactose sources for the glutaraldehyde-treated DEAE-cellulose immobilised cells and the results obtained are given in the accompanying diagram, FIG. 10. A 5% solution of lactose in 50 mM phosphate buffer, pH 7.0 (line 1); a solution of demineralised, ultrafiltered whey containing 48.95 mg per ml of lactose and at pH 6.55 (line 2); and skim milk containing 48.50 mg per ml of lactose and at pH 6.86 (line 3), are eluted through a column maintained at 60° C. containing glutaraldehyde-treated DEAE-cellulose immobilised cells (bed volume 41 ml). The flow rates are 28.3, 29.5 and 29.5 ml per hour respectively for the three substrates. The amount of glucose/galactose formed in samples of eluate is determined and the percentage hydrolysis calculated.