C([O-])([O-])=O.[K+].[K+].[CH3:7][N:8]([C:16]1[CH:25]=[CH:24][C:23]2[C:18](=[C:19]([CH2:26][CH2:27][NH:28][C:29](=[O:37])[CH2:30][C:31]3[CH:36]=[CH:35][CH:34]=[CH:33][CH:32]=3)[CH:20]=[CH:21][CH:22]=2)[CH:17]=1)[CH2:9][CH2:10][C:11]([O:13]CC)=[O:12].Cl>CO>[CH3:7][N:8]([C:16]1[CH:25]=[CH:24][C:23]2[C:18](=[C:19]([CH2:26][CH2:27][NH:28][C:29](=[O:37])[CH2:30][C:31]3[CH:36]=[CH:35][CH:34]=[CH:33][CH:32]=3)[CH:20]=[CH:21][CH:22]=2)[CH:17]=1)[CH2:9][CH2:10][C:11]([OH:13])=[O:12]An aqueous 0.5N solution of K2CO3 (10 ml) is added to the product obtained in Step A (4 mmol) dissolved in methanol (10 ml). When the reaction has ceased, the solution is acidified to pH 6–7 using 1N hydrochloric acid solution. The reaction mixture is extracted with dichloromethane.
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