o-Dianisidine dihydrochloride → Hexose

HRID 1255381

반응식

HRID 1255381 반응식
[OH:1][C@@H:2]1[O:10][C@H:9]([CH2:11][OH:12])[C@@H:7]([OH:8])[C@H:5]([OH:6])[C@H:3]1[OH:4].COC1C=C(C2C=CC(N)=C(OC)C=2)C=CC=1N.Cl.Cl>P([O-])([O-])([O-])=O.[Na+].[Na+].[Na+]>[CH2:11]([OH:12])[CH:9]1[O:10][CH:2]([OH:1])[CH:3]([OH:4])[CH:5]([OH:6])[CH:7]1[OH:8]

참가 물질

4

반응물 2

HCID 62311o-Dianisidine dihydrochlorideHCID 64689Beta-D-Glucose

생성물 1

HCID 206Hexose

시약·촉매·용매 1

HCID 24243Trisodium Phosphate용매2회

실험 절차

The assay was based on the method described by Sullivan and Ikawa (Biochimica et Biophysica Acta, 1973, 309:11-22), but modified to run in microtiter plates. An assay mixture contained 150 μl β-D-glucose (0.1 M in 0.1 M sodium phosphate buffer, pH 6.3), 120 μl 0.1 M sodium phosphate buffer, pH 6.3, 10 μl o-dianisidine-dihydrochloride (Sigma D-3252, 3.0 mg/ml in H2O), 10 μl peroxidase (POD) (Sigma P-8125, 0.1 ml in 0.1 M sodium phosphate buffer, pH 6.3) and 10 μl enzyme (HOX) solution. Blanks were made by adding buffer in place of enzyme solution.

후처리

  1. additionby adding buffer in place of enzyme solution

출처 및 근거

ORD 기록
ord-ac4e11fff7634ccc90f49a0aade7629e
출처 데이터셋
uspto-grants-2013_06
특허 번호
US08460723B2

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