N-Acetyl-DL-methionine → L-Methionine

HRID 127999

반응식

HRID 127999 반응식
C([NH:4][CH:5]([C:10]([OH:12])=[O:11])[CH2:6][CH2:7][S:8][CH3:9])(=O)C>[Co](Cl)Cl.O>[NH2:4][C@H:5]([C:10]([OH:12])=[O:11])[CH2:6][CH2:7][S:8][CH3:9]

참가 물질

4

반응물 1

HCID 6180N-Acetyl-DL-methionine

생성물 1

HCID 6137L-Methionine

시약·촉매·용매 2

HCID 24288Cobalt(II) chloride촉매HCID 962Water용매

반응 조건

온도
37 °C

실험 절차

The enzymatic activity of the acylase in solution, before and after fixing, is determined by adding 0.1 ml. of the enzyme solution to 5 ml. of an 0.4 weight percent solution of N-acetyl-DL-methionine in 0.1 M TRIS buffer, of pH 8, containing 5·10-4 mols of cobalt chloride. The mixture is maintained for 30 minutes at 37° C., whereupon the reaction is stopped by immersing the receptacle in boiling water for 3 minutes. After cooling, the L-methionine formed is determined.

후처리

  1. additionby adding 0.1 ml
  2. customfor 3 minutes
  3. temperatureAfter cooling

출처 및 근거

ORD 기록
ord-cebb56e106ae4cdaa34d3fb8f0a26a08
출처 데이터셋
uspto-grants-1981_03
특허 번호
US04258133

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