Panose → Panose

HRID 1353992노트

반응식

HRID 1353992 반응식
[CH2:1]([OH:34])[C@H:2]1[O:7][C@H:6]([O:8][CH2:9][C@H:10]2[O:15][C@H:14]([O:16][C@@H:17]([C@H:22]([OH:27])[C@@H:23]([OH:26])[CH:24]=[O:25])[C@H:18]([OH:21])[CH2:19][OH:20])[C@H:13]([OH:28])[C@@H:12]([OH:29])[C@@H:11]2[OH:30])[C@H:5]([OH:31])[C@@H:4]([OH:32])[C@@H:3]1[OH:33]>C([O-])(=O)C>[O:8]=[CH:6][C@@H:5]([C@H:4]([C@@H:3]([C@@H:2]([CH2:1][OH:34])[OH:7])[OH:33])[OH:32])[OH:31].[CH2:1]([OH:34])[C@H:2]1[O:7][C@H:6]([O:8][CH2:9][C@H:10]2[O:15][C@H:14]([O:16][C@@H:17]([C@H:22]([OH:27])[C@@H:23]([OH:26])[CH:24]=[O:25])[C@H:18]([OH:21])[CH2:19][OH:20])[C@H:13]([OH:28])[C@@H:12]([OH:29])[C@@H:11]2[OH:30])[C@H:5]([OH:31])[C@@H:4]([OH:32])[C@@H:3]1[OH:33]

참가 물질

4

반응물 1

HCID 94448Panose2회

생성물 2

HCID 94448PanoseHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

시약·촉매·용매 1

HCID 175Acetate용매

실험 절차

The enzymatic activity of α-isomaltosyl-transferring enzyme can be assayed as follows: Dissolve panose in 100 mm acetate buffer (pH 6.0) to give a concentration of 2% (w/v) f or a substrate solution, add 0.5 ml of an enzyme solution to 0.5 ml of the substrate solution, and keep the mixture at 35° C. for 30 min to proceed the formation of cyclotetrasaccharide from panose. In the reaction system, glucose is formed together with cyclotetrasaccharide from panose. After the reaction, boil the reaction mixture for 10 min to suspend the reaction. Subject the resulting reaction mixture to the glucose oxidase method to quantify the glucose formed in the reaction mixture. In the present invention, one unit activity of α-isomaltosyl-transferring enzyme is defined as the enzyme amount that forms one micromole of glucose per minute under the above enzymatic reaction conditions.

후처리

  1. customto give a concentration of 2% (w/v) f
  2. customat 35° C.
  3. customfor 30 min

출처 및 근거

ORD 기록
ord-830cf9066441413db5f6eab4ca695e89
출처 데이터셋
uspto-grants-2007_05
특허 번호
US07223570B2

반응 노트

불러오는 중…