Pyridoxal hydrochloride → Pyridoxal

HRID 1354531

반응식

HRID 1354531 반응식
CC([O-])=O.[Na+].[CH3:6][C:7]1[C:12]([OH:13])=[C:11]([CH:14]=[O:15])[C:10]([CH2:16][OH:17])=[CH:9][N:8]=1.Cl.[BH3-]C#N.[Na+]>O>[CH3:6][C:7]1[N:8]=[CH:9][C:10]([CH2:16][OH:17])=[C:11]([CH:14]=[O:15])[C:12]=1[OH:13]

참가 물질

5

반응물 3

HCID 6171Pyridoxal hydrochlorideHCID 517045Sodium AcetateHCID 5003444Sodium cyanoborohydride

생성물 1

HCID 1050Pyridoxal

시약·촉매·용매 1

HCID 962Water용매

실험 절차

PNA oligomer SEQ ID NO:63 or PNA oligomer SEQ ID NO:64 is treated according to the procedure of Example 62 to convert the N—PHTH group to the free amine. The resulting PNA oligomer is dissolved in 100 microliters of water, and 100 ml of 1M NaOAc buffer (pH 5.0) is added followed by 5 mg of pyridoxal hydrochloride (24 μmols) and 50 μl of 60 mM NaCNBH3 solution. The solution is vortexed, left overnight, and is then passed through a Sephadex G-25 column and further purified in an analytical HPLC column.

후처리

  1. additionPNA oligomer SEQ ID NO:63 or PNA oligomer SEQ ID NO:64 is treated
  2. customfurther purified in an analytical HPLC column

출처 및 근거

ORD 기록
ord-9822b87070304004a6400ec5607cd322
출처 데이터셋
uspto-grants-2007_05
특허 번호
US07223833B1

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