Huagongshe화공사 화학 정보 인쇄 보고서 · 연구 참고용
Creatine → Camp
참여 물질
| 역할 | 이름 | HCID | 투입량 |
|---|---|---|---|
| 반응물 | Creatine | HCID 586 | — |
| 반응물 | 3-Isobutyl-1-methylxanthine | HCID 3758 | — |
| 반응물 | 5'-Atp | HCID 5957 | — |
| 반응물 | 2-[Carbamimidoyl(methyl)amino]acetic acid;phosphoric acid | HCID 18629879 | — |
| 반응물 | Guanosine Triphosphate | HCID 135398633 | — |
| 용매 | Tris(hydroxymethyl)aminomethane | HCID 6503 | — |
| 생성물 | Camp | HCID 6076 | — |
반응 단계
Membranes are incubated in 25 mM Tris (pH 7.6), 0.2% BSA, 2.6 mM Mg, 0.8 mM ATP, 0.1 mM GTP, 5 mM creatine phosphate, 50 U/ml creatine kinase, 0.2 mM IBMX at 32° C. Prospective ligands are added and incubation is continued for another 15 minutes. The amount of cAMP produced is assayed using an fluorescent immunoassay method.
출처 및 근거
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 특허 번호 | US07655396B1 |
| 출처 데이터셋 | uspto-grants-2010_02 |
| ORD ID | ord-7ecdaa8541524bb59512d24ab4b045aa |
| 출처 메모 | uspto-grants-2010_02 |