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HRID1441207

反应详情

EQUATION

反应方程式

HRID 1441207 的结构方程式

REACTANTS

反应物

5
结构：CN(CC(=O)O)C(=N)N
HCID586

Creatine

C4H9N3O2

结构：CC(C)Cn1c(=O)n(C)c(=O)c2[nH]cnc21
HCID3758

Isobutylmethylxanthine

C10H14N4O2

结构：Nc1ncnc2c1ncn2[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]1O
HCID5957

Adenosine triphosphate

C10H16N5O13P3

结构：CN(CC(=O)O)C(=N)N.O=P(O)(O)O
HCID18629879

未命名化合物

结构：Nc1nc2c(ncn2[C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]2O)c(=O)[nH]1
HCID135398633

Guanosine triphosphate

C10H16N5O14P3

PRODUCTS

生成物

1
结构：Nc1ncnc2c1ncn2[C@@H]1O[C@@H]2COP(=O)(O)O[C@H]2[C@H]1O
HCID6076

Cyclic AMP

C10H12N5O6P

AUXILIARIES

试剂、催化剂与溶剂

1
结构：NC(CO)(CO)CO
溶剂HCID6503

Tromethamine

C4H11NO3

PROCEDURE

实验过程

Membranes are incubated in 25 mM Tris (pH 7.6), 0.2% BSA, 2.6 mM Mg, 0.8 mM ATP, 0.1 mM GTP, 5 mM creatine phosphate, 50 U/ml creatine kinase, 0.2 mM IBMX at 32° C. Prospective ligands are added and incubation is continued for another 15 minutes. The amount of cAMP produced is assayed using an fluorescent immunoassay method.

WORKUP

后处理

  1. customat 32° C
  2. additionProspective ligands are added